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Random (FR26RV-N) : 5’GCC GGA GCT CTG CAG ATA TC N NNN NN 3’

Random (FR26RV-N) : 5’GCC GGA GCT CTG CAG ATA TC N NNN NN 3’. 3’ primer (FR40RV-T) : 5’GCC GGA GCT CTG CAG ATA TC TTT TTT TTT TTT TTT TTT TT 3’. Virus specific 5’primer(e.g. rhinovirus) : 5’GCC GGA GCT CTG CAG ATA TC TTA AAA CTG G 3’. (AAAAAA)n. A. Viral RNA. Reverse Transcription

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Random (FR26RV-N) : 5’GCC GGA GCT CTG CAG ATA TC N NNN NN 3’

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  1. Random (FR26RV-N): 5’GCC GGA GCT CTG CAG ATA TCN NNN NN 3’ 3’ primer (FR40RV-T): 5’GCC GGA GCT CTG CAG ATA TC TTT TTT TTT TTT TTT TTT TT 3’ Virus specific 5’primer(e.g. rhinovirus): 5’GCC GGA GCT CTG CAG ATA TC TTA AAA CTG G 3’ (AAAAAA)n A Viral RNA Reverse Transcription (using random and 3’ primers) Single- stranded cDNA B Klenow Reaction (using random and 5’ primers) Double- stranded cDNA C PCR and A-tailing A A A A A A A A PCR amplicons A A D A A A A A A TA Cloning of 500 – 1000 nt amplicons and sequencing

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