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Chapter 49 – THE HELIX-TO-COIL TRANSITION IN DNA

Chapter 49 – THE HELIX-TO-COIL TRANSITION IN DNA. 49:5. A HELIX-TO-COIL TRANSITION MODEL. 49:6. APPLICATION OF THE MODEL TO UV ABSORPTION DATA. N H helical unit. n helical sequence. coil sequence. 49:5. A HELIX-TO-COIL TRANSITION MODEL. Partition for melting of 1 helical unit:.

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Chapter 49 – THE HELIX-TO-COIL TRANSITION IN DNA

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  1. Chapter 49 – THE HELIX-TO-COIL TRANSITION IN DNA 49:5. A HELIX-TO-COIL TRANSITION MODEL 49:6. APPLICATION OF THE MODEL TO UV ABSORPTION DATA

  2. NH helical unit n helical sequence coil sequence 49:5. A HELIX-TO-COIL TRANSITION MODEL Partition for melting of 1 helical unit: Partition for melting of 1 helical sequence: Partition function:

  3. 49:5. A HELIX-TO-COIL TRANSITION MODEL Diagonalize matrix: Partition function becomes: Eigenvalues: Fraction of units in the helical state: Relative number of helical sequences: Average number of helical units per helical sequence: H melting temperature:

  4. 49:6. APPLICATION OF THE MODEL TO UV ABSORPTION DATA Estimate: Fit of UV data yields: Enthalpy of melting: There are 29 sequences per 1000 base units

  5. COMMENTS -- Investigated salmon DNA in d-ethylene glycol. -- UV absorption spectroscopy monitors amine-base stacking in the DNA helical phase. -- It can detect for helix-to-melt transition. -- A simple model describes this melting transition.

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