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Video Tutorial on Chromatography, Paper, Thin Layer, Column Chromatography, HPLC and GLC. . http://lawrencekok.blogspot.com. Prepared by Lawrence Kok. Click here to view TLC and Column Chromatography. Separation using TLC. TLC techniques step by step. Column Chromatography.

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  1. Video Tutorial on Chromatography, Paper, Thin Layer, Column Chromatography, HPLC and GLC. http://lawrencekok.blogspot.com Prepared by Lawrence Kok

  2. Click here to view TLC and Column Chromatography Separation using TLC TLC techniques step by step Column Chromatography Column chromatography separation

  3. Click here to view HPLC and Gas Liquid Chromatography HPLC works Gas Liquid Chromatography works

  4. Chromatography Partition Chromatography vs Adsorption Chromatography

  5. Partition Chromatography Adsorption Chromatography 1 Pic taken from:http://www.liv.ac.uk/~agmclen/Medpracs/practical_4/theory_4.html

  6. Mobile phase - Liquid Mobile phase - Liquid Stationary phase - Liquid Stationary phase - Solid Pic taken from:http://www.liv.ac.uk/~agmclen/Medpracs/practical_4/theory_4.html

  7. Partition Chromatography Adsorption Chromatography 2 Distribution of X, Y and Z between two liquid phases Adsorption of X, Y and Z onto SOLID phase

  8. Type of chromatography

  9. Type of chromatography

  10. Type of chromatography Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase) Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase) Stationary phase – Solid silica SiO2 or alumina AI2O3 packed in column (solid phase)

  11. Type of chromatography Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase) Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase) Stationary phase – Solid silica SiO2 or alumina AI2O3 packed in column (solid phase) Mobile phase is liquid solvent Mobile phase is liquid solvent Mobile phase is liquid solvent

  12. Type of chromatography Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase) Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase) Stationary phase – Solid silica SiO2 or alumina AI2O3 packed in column (solid phase) Mobile phase is liquid solvent Mobile phase is liquid solvent Mobile phase is liquid solvent Solvent moves by capillary action Partition/Distribution of solute between 2 liquid phase Adsorption of solute onto Stationary solid phase Adsorption of solute on Stationary phase - strongly adsorp – travel slowly (eluted last) - weakly adsorp – travel fast (eluted first)

  13. Separated components measured using Rf values 1 2

  14. Click here to view Separation using TLC TLC techniques step by step Column Chromatography Column chromatography separation

  15. Type of chromatography

  16. Type of chromatography We also have HPLC and GLC

  17. High Performance Liquid Chromatography Gas Liquid Chromatography Picfrom:http://www.comsol.com/stories/waters_corp_hplc_systems/full/ Picfrom:http://www.sfu.ca/bisc/bisc-429/GLC.html

  18. High Performance Liquid Chromatography Gas Liquid Chromatography

  19. Click here to view HPLC works Gas Liquid Chromatography works

  20. Summary Paper Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid) Thin Layer Chromatography - Adsorption Chromatography - Mobile phase (Liquid) - Stationary phase (Solid) Column Chromatography - Adsorption Chromatography - Mobile phase (Liquid) - Stationary phase (Solid) High Performance Liquid Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid) Gas Liquid Chromatography – Partition Chromatography - Mobile phase (Gas) - Stationary phase (Liquid)

  21. Acknowledgements Thanks to source of pictures and video used in this presentation Thanks to Creative Commons for excellent contribution on licenses http://creativecommons.org/licenses/ Prepared by Lawrence Kok Check out more video tutorials from my site and hope you enjoy this tutorial http://lawrencekok.blogspot.com

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