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Learn how to locate the mouse ortholog of the human SSBP4 gene on Ensembl without a BLAST search. Follow the step-by-step guide provided to easily identify the mouse ortholog.
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Genomic Database - Ensembl Ka-Lok Ng Department of Bioinformatics Asia University
Ensemblhttp://www.ensembl.org/index.html • Worked example • The human protein single-stranded DNA binding protein 4, SSBP4. How can one find the mouse ortholog at Ensemble, without doing a BLAST search. • Ans. From the Ensembl human genome browser, search for the SSBP4 gene.
It returns Ensembl Gene: ENSG00000130511 (RefSeq NM_032627.2). Following the link to GeneView in the Orthologue Predication section are the results of sequence comparison between the human gene and genes from other organisms. The mouse gene ENSMUSG00000070003 is a best reciprocal hit to the human. This mouse gene (growth differentiation factor 15) is a putative ortholog of the human SSBP4 corresponding to RefSeq. NM_133772.1 (see the Similarity Section).
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