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Seahawk: How to use existing data with MOBY

Seahawk: How to use existing data with MOBY. Paul Gordon University of Calgary. Motivation. Professor: “I found a semi-automated way to do hundreds of BLASTs…I get my grad student to do them on the NCBI Web site, so it’s automated for me. ” Isn’t there a better way?.

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Seahawk: How to use existing data with MOBY

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  1. Seahawk: How to useexisting data with MOBY Paul Gordon University of Calgary

  2. Motivation • Professor: “I found a semi-automated way to do hundreds of BLASTs…I get my grad student to do them on the NCBI Web site, so it’s automated for me.” • Isn’t there a better way?

  3. Larry Wall’s “Virtues of a Programmer” HUBRIS IMPATIENCE LAZINESS

  4. Objectives • Appreciate the need for data and online service standardization • Incorporate multiple online resources into a pipeline • Use Seahawk to automate on-line analysis you do manually, repeatedly

  5. How software works… (Gene name, DNA sequence, QTL…) Parameters/Input Functions/ Rules Results/ Output (article, allele,…)

  6. DNASequence NCBI_gi Sequence_Alignment

  7. What is Seahawk? • Data-centric display: services are just links • Multi-tabbed: multi-task in your analysis

  8. How do I run a service? • If no “…” in description, the service runs just by clicking the popup label • If “…”, the service’s extra parameters can be set • Control+click submits using default params

  9. Take the output of the U of C service and send it to iHOP, then send its output to DDBJ’s service…

  10. How can we use non-Moby services? • Impatience: I don’t want to wait for someone to create a Moby equivalent… • Seahawk lets you define the input and output of the existing resources in terms of the Moby standard. • 4 easy steps!

  11. 1. Give Seahawk the original form • Find the WSDL document or Web form you want to use, then drag its URL from the browser’s location bar onto Seahawk. A new browser page will be launched. WSDL Web Service HTML Web form

  12. 2. Populating the form • Drag data links from Seahawk into the browser • Type in constant values Seahawk tracking the data type info!

  13. 3. Pick the output to return Autorecognized by Seahawk… …or a particular field

  14. 4. Annotate the new service • This helps you and others find it for reuse later

  15. Why Seahawk vs. Manual Analysis? • Laziness: Explicit iteration (“for each”) over… …existing services …or a Web form you just imported

  16. Managing wrapped services Tu deviens responsable pour toujours de ce que tu as apprivoisé.

  17. Seahawk Workflows • Document your analysis, be able to rerun it as a workflow. • Hubris: maybe modify the workflow to do other stuff later? (in Taverna)

  18. Seahawk as a stepping stone… • When you start opening data in new tabs, filtering pages (note highlight), using ‘previous input’ services, you’re writing non-trivial workflows!

  19. General Tips • Drag data to be imported into Seahawk onto the Clipboard, it’ll be recognized automatically. • Most items have a tooltip, just hold your mouse over the item of interest and wait. • It can take some time for a service list to be populated for a given type of data. You can try again later, and it should be much quicker.

  20. Summary • Use Seahawk for your analyses in order to: • Automate • Re-enact • Document • Learn Taverna Workflow Programming HUBRIS IMPATIENCE LAZINESS Web service/form wrapping For-each loops

  21. Feedback • Form provided to accompany the exercises • Help us improve the utility of the software! If you can not measure it, you can not improve it. – Lord Kelvin

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