1 / 6

Hose: Day 1, ~1 PM

Depicted on the following slides are water samples taken from scuba diving rinse tanks at a dive facility on Bonaire, Netherlands Antilles October, 2007, for 3 days.

ezra-quinn
Download Presentation

Hose: Day 1, ~1 PM

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Depicted on the following slides are water samples taken from scuba diving rinse tanks at a dive facility on Bonaire, Netherlands Antilles October, 2007, for 3 days. • Equipment rinse tank “A” was drained, cleaned with bleach and rinsed each morning, before filling with water and sampling (~8:00 AM) • Equipment rinse tank “B” was drained and rinsed each morning, before filling with water and sampling (~8:00 AM) • Water samples were taken from both equipment rinse tanks at ~1:00 PM and ~5:00 PM • Water samples were taken from a regulator rinse tank daily at ~8:00 AM, ~1:00 PM and ~5:00 PM, but we did not know when this tank was drained and refilled (by dive facility personnel and other divers) • Water samples were taken daily from the hose used to fill all rinse tanks • On two days water samples were taken from buckets on dive boats used to rinse/store masks • 0.25 ml of each water sample was spread on Terrific broth-agar plates; plates were incubated at 37oC overnight and photographed

  2. On all days, water samples from the hose (throughout the day) and from equipment rinse tanks “A” and “B” at ~8:00 AM, after draining, cleaning with bleach (“A”) or not cleaning with bleach (“B”), rinsing and refilling did not produce any bacterial colonies. Representative plates are shown below. Hose: Day 1, ~1 PM Hose: Day 2, ~8 AM Hose: Day 3, ~8 AM Tank “B”: Day 1, ~8 AM Tank “A”: Day 3, ~8 AM

  3. Day 1 Regulator tank: ~5 PM had no colonies (not shown) Regulator tank: ~8 AM Regulator tank: ~1 PM Tank “B”: ~5 PM Tank “B”: ~1 PM Tank “A”: ~1 PM Tank “A”: ~5 PM

  4. Day 2 Regulator tank: ~8 AM Regulator tank: ~5 PM Regulator tank: ~1 PM Tank “B”: ~5 PM Tank “A”: ~5 PM Tank “A”: ~1 PM Tank “B”: ~1 PM

  5. Day 3 Regulator tank: ~8 AM Regulator tank: ~1 PM Regulator tank: ~5 PM Tank “B”: ~5 PM was empty and samples were not taken Tank “A”: ~1 PM Tank “B”: ~1 PM Tank “A”: ~5 PM, while filling

  6. Mask rinse/storage tank: ~4 PM Day 1 Day 2

More Related