FACS Staining Protocol

1. Best Antibody Manufacturer That Provides Top Quality FACS and Intracellular Staining Protocol Flow cytometry is a popular cell biology technique that uses laser-based technology to sort, count and profile cells in a heterogeneous fluid mixture and it is a powerful tool that allows multi parametric analysis of both physical and chemical characteristics up to thousands of particles per second. This flow cytometry analysis makes it rapid and quantitative method for analysis and purification of cells in suspension and this technique also allows us to determine the phenotype and function and you can even sort live cells. Today, the terms flow cytometry and FACS are often used to describe the laser-based biophysical technique in which cell and other particles are passed through a laser light beam in a single file fashion. The electronic detection apparatus measures the interaction with light as fluorescence intensity and light scatter. What are FACS protocols? The term FACS is an abbreviation for “Fluorescence Activated Cell Sorting” and this FACS analysis is a flow symmetry technique that further adds a degree of functionality. Just like in conventional flow fluorescent signal data are collected, FACS protocols allow us to collect data on and to sort a biological sample by an endless number of different parameters antibodies labelled with fluorescent conjugates. The FACS technology is useful for a wide range of scientific fields from research to clinical as this used to physically heterogeneous mixture of cells into different populations. cytometry and by utilising sort a FACS is used for the analysis of whole human blood for diagnosing various diseases and sorting different blood cells for transplantations. FACS is also most commonly used in sorting and analysis of cell lines for various biological assays and also used in the analysis of murine blood to identify transgenic or knockout animals. https://www.facs-analysis.com/

2. Intracellular Staining Procedure in addition with FACS analysisis used to directly measure the antigens such as transcription factors and cytokines present inside the cell nucleus or cell cytoplasm and this procedure requires fixation and permeabilization of cells. This Intracellular Staining Protocol allows direct measurement of the functional activity of any cell of interest present in the blood without for the separation and this method can also be utilised for detection of the target protein. Therefore if you are looking for best antibody manufacturer that specialises in providing high- sensitivity, high-specificity compatibility antibodies then opting for Boster is the best choice to go with. We have years of experience in manufacturing antibodies that are thoroughly validated for numerous applications including IHC, WB, ELISA and flow cytometry. We strive to provide the best customer service and has earned the trust of scientists and researchers worldwide. For more details to know about BOSTER antibody and ELISA experts please visit our website here: https://www.facs-analysis.com. https://www.facs-analysis.com/