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∆Ct

∆Ct. + 7 1 -. NF- κ B concentration (fM).

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∆Ct

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  1. ∆Ct + 7 1 - NF-κB concentration (fM) Supplemental Data Fig. 2. NF-κB concentration-dependent relative Ct values (∆Ct). The solid red line is a linear regression fit (R2 =0.999) to the ∆Ct values. Insert is the amplification plots of real-time PCR for the 2 nM NF-κB probe in the presence of increasing amounts of NF-κB. The final concentrations of NF-κB were 10 fM (curve 1), 100 fM (curve 2), 1 pM (curve 3), 10 pM (curve 4), 100 pM (curve 5), 1 nM (curve 6), and 2 nM (curve 7), respectively. ‘+’ and ‘-’ indicate positive and negative controls, respectively.

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