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FLIM

FLIM. Fluorescence Life-Time Imaging Microscopy. ESF projekts Biofotonikas pētījumu grupa Nr.2009/0211/1DP/1.1.1.2.0/09/APIA/VIAA/077. Inesa Ferulova. 15 – 17.02.2012 - Daugavpils. DAUGAVPILS UNIVERSITĀTE . Dabaszinātņu un matemātikas fakultāte G . Liberta Inovatīvās mikroskopijas centrs.

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FLIM

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  1. FLIM Fluorescence Life-Time Imaging Microscopy ESF projekts Biofotonikas pētījumu grupa Nr.2009/0211/1DP/1.1.1.2.0/09/APIA/VIAA/077 InesaFerulova

  2. 15 – 17.02.2012 - Daugavpils DAUGAVPILS UNIVERSITĀTE Dabaszinātņu un matemātikas fakultāte G. Liberta Inovatīvās mikroskopijas centrs

  3. FLIM iekārta/shema Wavelength: 690 nm – 1040 nm Power: 2.6 W Repetition rate: 80 MHz • Wavwlength, Power: • 633 nm, 15 mW, 4 mW • 458 nm, 200mW, 30mW • 476 nm, 200mW, 30mW • 488 nm, 200mW, 30mW • 496 nm, 200mW, 30mW • 514 nm, 200mW, 30mW

  4. FLIM x,y plaknē x,yplaknē Fluorescence x,z plaknē

  5. Iekārtas Galvenās Sastāvdaļas • Piko sekunžu lāzers • Fotonu skaitītājs • TCSPC (Time-correlated single photon counting)/Pārveidotājs • Sinhronizators / no lāzera uz pārveidotāju dod signālu ka sakas jauns mērījums Time-correlated single photon counting

  6. FLIFluorescences Life-Time Imaging Lasercontroller interface Pico-second laser Opticalfiber SimpleTau 130 - Lifetimeanalyzer system Sample PC equippedwithsoftware

  7. Pico-second Laser PiLas • PiLascontrol EIG1000AF - repetition rate: 80 MHz Advanced Laser Diode Systems A.L.S. GmbH • Simple-Tau 130: • Ultra-fasttime-correlatedsinglephotoncountingsystembasedon SPC-130, consistingof: • NotebookComputerwithsoftware • 4 slot PCI-BoxwithconnectioncableandInterface-cardfornotebookcomputer • DCC-100 DetectorController • SPC-130 TCSPC Module • Photoncounter HPM-100-40-CMOUNT: • Hybrid PMT Detector Head for Photon Counting, 300-720nm. • Becker & HicklGmbH

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