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A PILOT STUDY OF BIOMONITORING AMBIENT GENOTOXIC EXPOSURE OF CHILDREN

A PILOT STUDY OF BIOMONITORING AMBIENT GENOTOXIC EXPOSURE OF CHILDREN. L. Anna , B. Schoket, E. Győrffy, E. Erdei, P. Rudnai National Institute of Environmental Health, József Fodor National Centre for Public Health, Budapest, Hungary. AIM OF THE STUDY.

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A PILOT STUDY OF BIOMONITORING AMBIENT GENOTOXIC EXPOSURE OF CHILDREN

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  1. A PILOT STUDY OF BIOMONITORING AMBIENT GENOTOXIC EXPOSUREOF CHILDREN L. Anna, B. Schoket, E. Győrffy, E. Erdei, P. Rudnai National Institute of Environmental Health, József Fodor National Centre for Public Health, Budapest, Hungary

  2. AIM OF THE STUDY • To assess genotoxic exposure of children to air pollution • To explore the suitability of aromatic DNA adducts as a biomarker of genotoxic exposure of children

  3. EPIDEMIOLOGICAL BACKGROUND OF THE STUDY • Investigation of the respiratory health of 1800 children of 8 to 10 years of age (Nov-Dec 1998) •Six towns in Hungary, three of those with elevated air pollution •Exposure and health questionnaires filled in by the parents

  4. STUDY POPULATION FOR THE BIOMONITORING STUDY N = 105 Exposure categories Polluted townsControl towns ETS & Traffic ETS & Traffic ETS only ETS only Traffic only Traffic only No ETS & No traffic No ETS & No traffic

  5. METHODOLOGY • Isolation of DNA from frozen whole blood (ca. 1.5 ml) • Detection of aromatic DNA adducts by 32P-postlabelling • Radioactivity measurement by electronic autoradiograph • Statistical analyses by Fisher’s exact test and Mann-Whitney U test

  6. STRUCTURE OF BENZO[A]PYRENE DIOL-EPOXIDE-DNA ADDUCT

  7. TYPICAL TWO-DIMENSIONAL DNA ADDUCT MAPS FROM CHILDREN’S BLOOD a b c d e f Blank

  8. PERCENTAGE OF BLOOD SAMPLES WITH DNA ADDUCT SPOTS IN THEPOLLUTED AND CONTROL TOWNS

  9. PERCENTAGE OF BLOOD SAMPLES WITHDNA ADDUCT SPOTS POLLUTED TOWNS CONTROL TOWNS

  10. ODDS RATIOS FOR DNA ADDUCT FORMATIONDUE TO ELEVATED AIR POLLUTION(Main adduct spot)

  11. INDIVIDUAL VARIATION IN AROMATIC DNA ADDUCT LEVELS IN BLOOD SAMPLES FROM CHILDREN Values for the main adduct spot Polluted towns Control towns P=0.02 for the Means

  12. AROMATIC DNA ADDUCT LEVELS IN BLOOD SAMPLES FROM CHILDREN Values for the main spot (Mean ± SD) Polluted towns Control towns P=0.002 * *

  13. CONCLUSIONS •DNA adducts were detectable from frozen whole blood •Detection limit was as low as 0.02-0.07 adducts in 108 nucleotides •Two characteristic distinct adduct spots were related to the exposure •Statistically significant difference was observed in DNA damage between the highest and lowest exposure categories • Aromatic DNA adduct was a sensitive biomarker of genotoxic exposure of children to air pollution

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