Introduction pg. ix – xi

1. Introduction pg. ix – xi Lab 2

2. General Lab Safety General Work Environment: Aisles and passages kept clear (3 feet minimum) Exits: Identify Exit locations Rehearse how to evacuate incase of fire; where to assemble, take roll. Emergency Planning: Fire extinguisher location Eyewash, shower and safety supplies First aid kits readily accessible Gas: Master turn-off valve Spill clean-up kits

3. The Right to Know Required information and postings Material Safety Data Sheets Signage Locations of hazardous material Locations of safe handling equipment All reagents labeled

4. In this lab you will be working with living organisms that may or may not be considered living organisms, therefore, all microbes should be treated as a potential pathogen. • You must adhere to strict lab safety guidelines to ensure the safety of yourself, your classmates, and your family and friends at home.

5. Species List

6. Biosafety Levels (BSL) • BSL 1 - BSL-1 is appropriate for working with microorganisms that are not known to cause disease in healthy human humans. This is the type of laboratory found in municipal water-testing laboratories, in high schools, and in some community colleges teaching introductory microbiology classes, where the agents are not considered hazardous. • BSL 2 - The agents manipulated at BSL-2 are often ones to which the workers have had exposure to in the community, often as children, and to which they have already experienced an immune response. These agents are generally transmissible following ingestion, exposure of mucous membranes, or intra-dermal exposure. Eating, drinking and smoking are prohibited in BSL-2 laboratories, and extreme precautions are taken while handling needles and other sharp instruments. • From “The 123s of Laboratory Biosafety”

7. General Lab Safety Rules See Handout Sign Agreement

8. Good Practices Label all materials with your name, date, and any other applicable information (e.g., media, organism, etc.). Dispose of wastes in their proper containers. Return all chemicals, reagents, cultures, and glassware to their appropriate places. Observe chemical compatibilities. Do not pour biohazardous fluids down the sink. Glassware should be washed after use and put away All shelves should be labeled and un-cluttered Flame transfer loops, wires, or needles before and immediately after use to transfer biological material. We use live bacteria!

9. Waste Disposal Prevent exposure to pathogens and hazards: Containers are compatible with waste: Non-contaminated glass :Broken Glass Boxes Contaminated broken glass: Sharps container Soiled but not contaminated gloves, aprons can go in the trash Contaminated gloves, aprons in bio-hazardous waste Bio-hazardous waste: Red bag in biohazard box Needles, lances, blades: Sharps container

10. Contacts Lab Manager: Joia Sherry ext. 3739 Or Tom Arnold ext. 3697 Facilities Manager: Mike Oun ext. 5381 Security: 1-800-519-5002 In case of fire or emergency dial 911