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40 35 30 25 20 15. standards no template control. A. B. 200 160 120 80 40 0 -40. Threshold Cycle. R 2 = 0.999 PCR Efficiency = 98.2% y = -3.367x + 38.019. Base line subtracted RFU.
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40 35 30 25 20 15 standards no template control A B 200 160 120 80 40 0 -40 Threshold Cycle R2 = 0.999 PCR Efficiency = 98.2% y = -3.367x + 38.019 Base line subtracted RFU 0 1 2 3 4 5 6 Log Copy Number 0 4 8 12 16 20 24 28 32 36 40 PCR Cycle Durham et al Online supplement Figure 1.