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unmethylated. M.SssI-methylated. 5x. 2x. 1x. 5x. 2x. 1x. 25x. 10x. 25x. 10x. 125x. 125x. M. 0. 0. M. (bp). 2000. 1500. 1000. 750. 500. 250.
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unmethylated M.SssI-methylated 5x 2x 1x 5x 2x 1x 25x 10x 25x 10x 125x 125x M 0 0 M (bp) 2000 1500 1000 750 500 250 Figure S1. Digestion of unmethylated and M.SssI-methylated pUP41 plasmid DNA with MvaI. pUP41 plasmid DNA was digested with FastDigest MvaI (Fermentas) at 37oC for 2 hours.Numbers above the lanes indicate dilutions of the commercial enzyme preparation used for the digestion. 0, undigested; M, 1 kb DNA Ladder (Fermentas).