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Why Cryogenic Grinding?

Why Cryogenic Grinding?. Many analytical samples are too flexible or sensitive to be ground at ambient temperatures Samples are subject to degrading Does not damage DNA or RNA. The Basics.

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Why Cryogenic Grinding?

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  1. Why Cryogenic Grinding? • Many analytical samples are too flexible or sensitive to be ground at ambient temperatures • Samples are subject to degrading • Does not damage DNA or RNA

  2. The Basics • Samples are chilled at Liquid Nitrogen (LN) temperatures and ground using a Magnetically-Driven Impactor • No mechanical linkages or bearings to fail • Sealed Grinding Vials ensure sample integrity • Touch screen display w/ stored procedures for rapid process replication and consistency

  3. Who Can Benefit From a Freezer/Mill®? • Forensic Labs – DNA identification • Medical Labs– genetic research, disease prevention • Hospital Labs – For genetic screening • Agricultural Labs – For crop development • Agricultural Veterinary Labs – For livestock breeding and health • Archealogy Labs – Ancient DNA extraction • Public Health and Environmental Monitoring Labs • QC Testing of Manufactured Goods • Electronics Companies – Grinding Electronic Components

  4. The Freezer/Mill® Family

  5. 6970EFM – Features & Benefits • Two Models Available • Single Grinding Chamber (6970M) • 1 Grinding Chamber + 1 Pre-Cooling Chamber • Dual Grinding Chambers (6970D) • Totally enclosed auto-fill system with no Liquid Nitrogen exposure • Increased through-put • Up to 8 small vials or 2 large vials(D model only)

  6. 6770 – Features & Benefits • Cost efficient • Runs one Micro-vial or small vial per run • Perfect for smaller samples (5 grams or less) • Additional area for pre-cooling samples • Unit has small counter footprint

  7. 6870 – Features & Benefits • Versatile large grinding chamber • Runs a variety of vials, ranging from micro vials through the large, even up to 4 of the small vials • Allows for wide range of sample sizes • Available with Auto-Fill, or without • Additional area for pre-cooling samples

  8. Freezer/Mill Accessories

  9. Freezer/MillVials & Vial Sets Vial Sizes Vial Materials • Standard Set • Stainless Steel End Plugs & Impactor with Polycarbonate Center Cylinder • Polycarbonate • Avoids metal contamination • Stainless Steel • Best for Polymer applications • Cr-Free • For RoHS/WEEE appliactions

  10. Freezer/MillAuto-Extractor 6814 Auto-Extractor™ Works with Small and Large size vials Increases throughput Reduces operator effort

  11. Freezer/MillAccessory Packages 6870S • Includes: • 6754 Small Extractor • 6755 Vial Rack • 6806 Multi-Vial Adapter 6870M • Includes: • 6884 Mid-Size Adapter for 6804 Large Extractor • 6886 Mid-Size Grinding Vial Adapter 6870L • Includes: • 6804 Large Extractor • 6805 Vial Rack

  12. Freezer/MillLiquid Nitrogen Handling Safety • Cryogenic Gloves Liquid Nitrogen • Portable Dewars & Pressurized Tanks • Required for use with Auto-Fill units(6870 + Auto-Fill & 6970EFM)

  13. Samples, Application Notes & Resources

  14. Before & After… Leather Corn A Collection of our Before & After Samples Bone

  15. Application Notes • Extensive library of Freezer/Mill® applications Examples • SP005 – RNA Extraction from Cartilage Tissue Using Cryogenic Grinding • SP006– Preparation of Rice Husks for the Analysis of Their Contents • SP007– Grinding Polymers For Qualitative and Quantitative Analysis Also Available: Featured Articles Library

  16. Product Demonstration Videos

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