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VIBRIOS

VIBRIOS. By. Dr. Emad AbdElhameed Morad. Lecturer of Medical Microbiology and Immunology. Vibrio cholerae. Morphology. Gram negative comma shaped bacilli, motile with single terminal flagellum (darting motility). Cultural characters. Highly aerobic

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VIBRIOS

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  1. VIBRIOS By Dr. Emad AbdElhameed Morad Lecturer of Medical Microbiology and Immunology

  2. Vibrio cholerae

  3. Morphology Gram negative comma shaped bacilli, motile with single terminal flagellum (darting motility).

  4. Cultural characters Highly aerobic Growth is favoured by alkaline pH (8-9) On alkaline peptone water, forms surface pellicle within 8 hours. On TCBS, produce yellow colonies.

  5. Biochemical reactions Ferment glucose, maltose, mannite, sucrose with production of acid only. Oxidase positive Indole positive Cholera red reaction positive: Vibrio cholera is inoculated in nitrate peptone water. The bacteria reduces nitrate to nitrite. The bacteria also decomposes peptone to indole. Nitrite combines with indole forming nitroso-indole which gives red color on addition of strong acids.

  6. Sugar fermentation reaction

  7. Oxidase test

  8. Indole test

  9. Rice watery diarrhea

  10. Secondary case during an epidemic Laboratory diagnosis Cases are diagnosed by microscopic examination of stools for comma shaped bacilli with darting motility.

  11. First case in a non-endemic area Wet mount Gram stain Biochemical reactions The stool is inoculated in alkaline peptone water for 6-8h. Then, subculture is done on TCBS. The growing colonies are identified by: for darting motility. Gram negative comma shaped bacilli. sugar fermentation, oxidase, indole, cholera red reaction. with specific anti-O1 and 139 sera. Direct methods for detection of V. cholerae O1 and O139 include immunofluorescence and PCR for detection of cholera toxin gene. Agglutination tests

  12. GOOD LUCK

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