1 / 14

Selected and Differential Media

Selected and Differential Media. Define . Selective Media Differential Media Enriched Media. Blood Agar Plate (BAP). Differential Enriched Use Growth Hemolysis Alpha Beta Gamma. C-CNA. Selective Differential Results Gram (+) Colistin Naldixic acid Blood Hemolysis. MSA.

faxon
Download Presentation

Selected and Differential Media

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Selected and Differential Media

  2. Define • Selective Media • Differential Media • Enriched Media

  3. Blood Agar Plate (BAP) • Differential • Enriched • Use • Growth • Hemolysis • Alpha • Beta • Gamma

  4. C-CNA • Selective • Differential • Results • Gram (+) • Colistin • Naldixic acid • Blood • Hemolysis

  5. MSA • Selective • Differential • Results • Halotolerant (7.5% NaCl) • CH2O: Mannitol • pH • phenol red • Fermentation • Acid • Yellow

  6. MAC • Selective • Differential • Results • Gram (-) • CV • Bile Salts • Lactose • pH indicator • Neutral red indicator • Ferment = pink/red • No acid = colorless

  7. EMB • Selective • Differential • Results • Gram (-) • Eosin dye • MB dye • Lactose • Some acid = pink • Large acid = green • No acid = colorless

  8. Set Up • One plate for each group • Label quadrants as lab book indicates for each plate • Cultures on cart • Anaerobic Gas Pack • BAP • C-CNA • All others aerobic • 37°C

  9. BAP results • Sheep blood enriched • Growth most all microbes • Fastidious • Differential • GP Hemolysins • Hemolysis types • Gamma • Alpha • Beta

  10. Hemolysis No hemolysis

  11. C-CNA results • Selective • Growth GP • Colistin • Nalidixic acid • Differential • Hemolysis

  12. MSA results Selective 7.5% NaCl Staphylococcus sp Differential Mannitol fermentation phenol red  yellow

  13. MAC results • Selective: GN • CV • Bile Salts • Differential • Lactose fermentation • NF = colorless • Coliforms • Neutral red  pink

  14. EMB results • Selective: GN • Dyes • Eosin • MB • Differential • Lactose fermentation • NF = colorless • Acid production • Small = pink • Large = metallic green

More Related