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Cellulase enzyme

Cellulase enzyme. Introduction. EC Code = 3.2.1.4

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Cellulase enzyme

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  1. Cellulaseenzyme

  2. Introduction • EC Code =3.2.1.4 • Other name s: Avicelase- BETA-1,4-Endoglucan Hydrolase - BETA-1,4Glucanase- Cello dextrinase Carboxy methyl cellulase ENDO-1,4-BETA-D-Glucanase-ENDO-1,4-BETA-D-Glucanohydrolase- ENDO-1,4-BETA-Glucanase- Endo glucanase -Alkali cellulase- Pancellasess • Systematic name:1,4-(1,3:1,4)-beta-D-glucan,4-glucano hydrolase

  3. REACTION • 1-4-BETA-GLUCOSIDIC bond hydrolysis in cellulose,…

  4. cellulases • Endo-cellulase • Exo-cellulase • beta-glucosidase • Oxidative cellulase • Cellulose phosphorylase

  5. Cellulase sources • Bacteria • Fungi • Actinomycete • Protozoa • Some insects that digest wood • Some animals & plants • Not mammal

  6. Extraction • Dialysis • Filtration gel • Anionic resin exchange column • Using aceton

  7. Cellulase production

  8. Cellulase activity assay • Spectorophotometery • Reduced sugar • Substrate decrease • Solid surface • Propagation in substrate • Electrophoreses gel

  9. Cellulase application • Food industry • Agricultural industry • Paper making industry • Waste water treatment • Textile industry

  10. Enzymic hydrolysis rate

  11. Cellulase action mechanism

  12. Cellulase structure The crystal structure of the catalytic domain of CelCCA β strands and * helices in CelCCA.

  13. Topological diagram of the secondary structural elements of CelCCA. helices are shown as cylinders and β strands as arrows.

  14. Close up view of catalitic C-terminal extremlybof the barrel strands showing the arrangment of the 7 residues(red and yellow) along with the try (purple) at the intrancethe catalitic residues are red

  15. Thank for your patience

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