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Enhance the rate of transport of reactants to a reaction surface on the wall of a microchannel.

AC Electrokinetically Enhanced Surface Reactions. Enhance the rate of transport of reactants to a reaction surface on the wall of a microchannel. Generate swirling patterns in the fluid and thereby enhance the transport of the analyte to the reaction surface.

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Enhance the rate of transport of reactants to a reaction surface on the wall of a microchannel.

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  1. AC Electrokinetically Enhanced Surface Reactions Enhance the rate of transport of reactants to a reaction surface on the wall of a microchannel. Generate swirling patterns in the fluid and thereby enhance the transport of the analyte to the reaction surface. Biological immunoassays, which detect an analyte through their binding response to an antibody ligand, can use these flow patterns to great advantage 學生: 鄭宜肪 授課老師: 李旺龍 教授

  2. Electrostatic Current density Poisson’s equation Laplace’s equation

  3. Electrothermal and Dielectric Force and Electrothermal force For water:

  4. Joule Heating and Binding Reaction Navier-Stokes equations Q: heat source (a unit volume of fluid absorbs through Joule heating) Heat balance C: heat capacity k: the fluid’s thermal conductivity Convection and Diffusion c: concentration D: diffusion coefficient Rt: total surface concentration of antibody c: analyte concentration on the surface B: The portion concentration of the bounded molecules

  5. Applied Models

  6. Setting Boundary Expression Rt: total surface concentration of antibody c: analyte concentration on the surface B: The portion concentration of the bounded molecules

  7. Scalar Expression Setting Dielectric force induced thermal flow ω: frequency of voltage τ: relaxation time For water:

  8. Setting Surface Subdomain Expression

  9. Setting Boundary Variable

  10. Setting the Variable of Analyte Concentration on the Reaction Region

  11. Setting the Variable of Analyte Concentration on the Surface Reaction Region

  12. Setting the Variable of Analyte Concentration on the Reaction Region

  13. Sobdomain Integration Variable of Reaction Surface Setting B_total: Binding concentration of the reaction surface

  14. Electrostatic Subdomain Setting For water: εr=80

  15. Convection and Conduction Subdomain Setting Density of the fluid= 999 Heat capacity= 4.184e3 Heat source= σE2

  16. Navier-Stokes Subdomain Setting Density of the fluid= 999 Dynamic viscosity: 1.08e-3

  17. Diffusion Subdomain Setting Diffusion coefficient: 1e-11

  18. Surface Subdomain Setting of Reaction Rate Reaction rate

  19. Electrostatic Initial Value Setting of Solver Manager

  20. Electric Potential +5V -5V

  21. Initial Value Setting of Solver Manager

  22. Temperature Distribution -5V +5V

  23. Velocity Distribution Vortex generation

  24. Velocity Field Vortex generation

  25. Concentration Distribution During 1 Sec

  26. Concentration Distribution without Voltage Applied

  27. Concentration Distribution with Voltage Applied During 5 Sec Concentration decreasing Concentration increasing

  28. Δ: without voltage applied □: with voltage applied Binding Concentration of Reaction Surface of Time Variation between with and without Voltage Applied Enhanced binding rate when ac voltage applied!

  29. Simulation of Micro Fluorescence Active Cell Sorter (mFACS) A. Y. Fu1, C. Spence, A. Scherer, F. H. Arnold, and S. R. Quake, “microfabricated fluorescence-activated cell sorter”, Nature Biotech.,17(1999)1109-1111. Electroosmotic flow driven !

  30. Diffuse layer - - - + + + - - + + + + + - + + + + + + ɸ0 Stern layer Potential + + + + + + + - - - - - - - - - - Ion concentration C+ Distance from surface C- Distance from surface Electrical Double Layer

  31. Electroosmotic Flow electroosmotic velocity

  32. Theory and Equations Navier-Stokes equations Electric field Current density Current density Current density Current density electroosmotic velocity V=V0 Poisson’s equation Poisson’s equation Laplace’s equation Laplace’s equation

  33. Subdomain Setting for Stokes Flow

  34. Subdomain Setting for Conductive DC Model

  35. Stock’s Flow Boundary Condition

  36. Stock’s Flow Boundary Condition

  37. Stock’s Flow Boundary Condition

  38. Boundary Conditions of Conductive DC Model

  39. Electric Potential Ground 1000 V 1000 V 1000 V Ground

  40. Electric Field Distribution

  41. Flow Velocity Distribution Ground 1000 V 1000 V 1000 V Ground

  42. Flow Switching through Changed Voltage Location 1000 V Float 1000 V 1000 V Ground

  43. Flow Switching through Changed Voltage Location Ground 1000 V 1000 V Float 1000 V

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