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Polarization Toolbox Electro-Optical L-Format (EOLF) Polarization of Fluorescence Module

Polarization Toolbox Electro-Optical L-Format (EOLF) Polarization of Fluorescence Module Add Anisotropy and Circularly Polarized Luminescence Easily. Traditional L-Format Anisotropy. I HV. G =. Gain Correction:. I HH. I V V – GI VH. P =. I V V + GI VH. Polarization:. I V V – GI VH.

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Polarization Toolbox Electro-Optical L-Format (EOLF) Polarization of Fluorescence Module

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  1. Polarization Toolbox Electro-Optical L-Format (EOLF) Polarization of Fluorescence Module Add Anisotropy and Circularly Polarized Luminescence Easily

  2. Traditional L-Format Anisotropy

  3. IHV G = Gain Correction: IHH IV V – GIVH P = IV V + GIVH Polarization: IV V – GIVH r = Anisotropy: IV V + 2GIVH Traditional Calculation of Anisotropy and Polarization

  4. Anisotropy with New EOLF

  5. IV V – IVH Polarization: P = IV V + IVH IV V – IVH r = Anisotropy: IV V + 2IVH Calculation of Anisotropy and Polarization using an EOLF Module

  6. EOLF Advantages • No Gain Correction Factor (G-Factor) • No polarizer movement • Rapid switching between polarization states • supports high speed kinetic experiments

  7. EOLF Chamber, PEM version

  8. EOLF Chamber in a DM 45 Spectrofluorimeter

  9. Comparison of PEM and LCVR Properties

  10. Photon Counter Gating

  11. Polarization Anisotropy Excitation Anisotropy Spectrum of Rhodamine

  12. Fluorescein Spectra as a Function of Temperature temperature Increasing

  13. EOLF Also Supports CPL Circularly Polarized Luminescence (CPL) • Differential emission of left and right circularly polarized light • Fluorescence analog of circular dichroism (CD) • Sensitive to environmental changes of chiral fluorophores

  14. 30000 Left polarized 20000 Right polarized Intensity, counts 10000 0 570 580 600 610 620 630 590 Emission Wavelength, nm Left and Right Polarized Emission Spectra Europium(III) tris(trifluoromethylhydroxymethylene)-d-camphorate inDMSO

  15. CPL Spectrum of Europium Compound

  16. CPL with Polarized Excitation Use circular polarized excitation to photoselect one enantiomer in a racemic mixture

  17. US Patent Pending

  18. EOLF Adds Complementary Techniques to a Single Optical Bench

  19. With One EOLF, Measure: • Conformation characterization of proteins and other biological macromolecules • Equilibrium binding studies of macromolecules • Characterization of metal ligand complexes • Characterization of entantiameric mixtures

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