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Staining HAEMATOXYLIN & EOSIN

Staining HAEMATOXYLIN & EOSIN. Supervised by : Dr.Sherifa Prepared by: Reem Aldossari Lama Alabdi. H&E staining:

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Staining HAEMATOXYLIN & EOSIN

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  1. StainingHAEMATOXYLIN & EOSIN Supervised by : Dr.Sherifa Prepared by: Reem Aldossari Lama Alabdi

  2. H&E staining: • The best known and most widely used staining procedure, hematoxylin-eosin staining (H&E), uses hematoxylin solutions for nuclear staining and eosin solutions for cytoplasmic staining. • In the first step, the nuclei are stained with a hematoxylin solution. The nuclei stain blue, dark violet to black. • The second step is counterstaining with eosin Y or eosin B. • In this process cytoplasm, collagen, keratin stain red.

  3. H&E staining is the standard staining method used in histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal, inflammed,degenerative and pathological changes . • It is often possible to make a diagnosis based on the results of H&E staining, or it can be determined which special staining techniques are still necessary for subtyping.

  4. APPLICATION • Gives a general overview of the tissue structure. • Can be used as a diagnostic tool.

  5. Advantages of H&E staining • Combination of the two stains gives outstanding • results. • Ready to use reagents . • Easy to use. • Results in just two steps.

  6. Procedure for H & E Staining of Paraffin Sections: • 1-(Fixative: Any general fixative is adequate. Ex.formalin) • 2--Deparaffinization in : • 3 - labeledXylene I ( 1-3 minutes) • 4- EtOH (ethanol alcohol) 100% (2-3 minutes • 5-EtOH(ethanol alcohol) 96% (2-3 minutes) • 6-EtOH(ethanol alcohol) 80% (2-3 minutes) • 7-EtOH(ethanol alcohol) 70% (2-3 minutes) • 8-Dist. water, 2 minutes. • 9. Hematoxylin, approximately 10 minutes. 10-EtOH(ethanol alcohol) 70% (2-3 minutes) 11-EtOH(ethanol alcohol) 80% (2-3 minutes) 13-Eosin Y solution, 2 to 5 minutes. 16- Absolute 100% ethanol, 1-2 minutes. 18-Xylene I, 1-2 minutes. 21-Mounting with DPX • 12-EtOH(ethanol alcohol) 96% (2-3 minutes)

  7. Thank you

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