Introductiob to bacillus subtilis expression

1. Something you want to know about bacillus subtilis expression The Bacillus subtilis is a Gram-positive bacterium isolated from the soil originally. Although it has been applied in agriculture and traditional food technology for centuries, the great scientific advancement later revealed this incredible microorganism as a rod-shaped facultative anaerobe with a general length of 4-10 μm and 0.25-1.0 μm in diameter, which forms approximate 4.6 f L cell volume at stationary phase. As one of the best-studied model bacteria, the Bacillus subtilis manifests as an increasingly popular bacterial champion for more and more applications for heterologous protein expression among several widely used prokaryotic organisms to produce multiple enzymes and other functional protein productions, no matter in the basic research area or commercial industrial realm. Noticeable, the current application of B. subtilis in the industrial realm accounts for over 60 % of the commercial enzymes production for the following attractive characteristics: i. Excellent amenability for the small- and large-scale fermentation; ii. Complete basic characterization of multiple molecular mechanisms, such as its completely sequenced genome, transcription, translation, protein folding, secretion mechanism, and metabolic pathways; iii. Non-pathogenic and endotoxin-free microorganism labeled with a Generally Regarded as Safe (GRAS) status by the U.S. Food and Drug Administration; iv. Capable of expressing heterologous proteins (antibody, vaccine, functional peptide, etc.) without significant bias in codon usage and secreting them into extracellular culture media with high yield.