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Using the FST to Correlate Depressive-Like Behavior and Estrogen Levels in Three Strains of Mice

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Using the FST to Correlate Depressive-Like Behavior and Estrogen Levels in Three Strains of Mice

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  1. This presentation will probably involve audience discussion, which will create action items. Use PowerPoint to keep track of these action items during your presentation • In Slide Show, click on the right mouse button • Select “Meeting Minder” • Select the “Action Items” tab • Type in action items as they come up • Click OK to dismiss this box • This will automatically create an Action Item slide at the end of your presentation with your points entered. Using the FST to Correlate Depressive-Like Behavior and Estrogen Levels in Three Strains of Mice Kunwal Nasrullah Columbia University, College of Physicians and Surgeons D. Toran-Allerand M.D., Sc.D. Imam Nethrapalli, PhD

  2. Key Terms Depression-“symptoms meeting established clinical threshold criteria for depression as measured by validated questionnaires or standardized psychiatric interviews”¹vs. depressive-like behavior Forced Swimming Test (FST)-a behavior test that serves as a rodent model for depressive-like symptoms in humans. Estrogens- 17β-estradiol vs. 17α-estradiol Mice strains: BALB/c, 129/SvEv, and c57BL/6J Intact vs. Ovariectomized (OVX) vs. Adrenalectomized (ADRX)

  3. What I do… I am a double-blind scorer of mice FST conducted by my co-mentor, Imam Nethrapalli. Our ongoing project is to test the hypothesis that 17α-estradiol is produced locally in the brain and that it has antidepressant activities in mice.

  4. What I see… http://www.biobserve.com/products/fst/acquisition_movie.html Computer Image of FST http://62.193.209.148/en/content.php?content.66

  5. Things that I look for… During an FST scoring, three actions can be observed: • swimming • climbing/scratching along the sides of the container • floating/immobility: no active movement HINT: The surface of the water is a good indicator of movement or immobility.

  6. Possible Results My results can help to validate previous findings of my mentor. It is also possible that my results will negate the findings of my mentor. In either case, it is necessary to certify the antidepressant effects of 17α-estradiol in mice. These results will be used in a paper.

  7. Future Plans I will continue to score the FST relevant to the study and learn lab techniques related to these behavior studies. We will add results of an Open Field Test (a 3-by-3 feet square made of melamine which allows researchers to gauge the anxiety of mice and rats) to the results of the FST to support the lab’s work. Open Field Test http://www.biochemj.org/bj/389/0593/bj3890593f01.gif

  8. References (2007). Bioserve: solutions for yourscience. Retrieved August 2, 2007 from http://www.biobserve.com/products/fst/acquisition_movie.html. (2006). Cheshire wildlife trust. Retrieved August 2, 2007 fromhttp://www.wildlifetrust.org.uk/cheshire/IMAGES/watch_harvest_mice.jpg. ¹ Frasure-Smith N, Lesperance F, Talajic M, et al. Depression following myocardial infarction. Impact on 6-month survival. JAMA. 1993;270:1819–25. Porsolt et al., 1978 R.D. Porsolt, G. Anton, N. Blavet and M. Jalfre, Behavioural despair in rats: a new model sensitive to antidepressant treatments, European Journal of Pharmacology. 47 (1978), pp. 379–391. Toran-Allerand CD, Tinnikov AA, Singh RJ, Nethrapalli IS. 2005. 17alpha-estradiol: a brain-active estrogen? Endocrinology, 146:3843–3850. van Meer, P., & Raber, J. (2005). Mouse behavioral analysis in systems biology. Biochemical journal, 389. Retrieved August 1, 2007, from http://www.biochemj.org/bj/389/0593/bj3890593.htm. Villalba, Shannon. (2005). The behavior core. Retrieved August 1, 2007 from http://www.psychiatry.uc.edu/BehaviorCore/HTML/openfield.html.

  9. Acknowledgments • Dr. Sat • HCS • HCS staff • Dr. Toran-Allerand • Dr. Imam Nethrapalli • Caitlin • Columbia University, College of Physicians and Surgeons • MSKCC • And you!

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