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Gregor 2007

Gregor 2007. Use GFP- Bcd to further 2005 work Use time-lapse two-photon microscopy Fast and sensitive See Bcd on nuclei and anterior to posterior gradient. Gregor 2007. Fluorescence increases with time . Quick Summary.

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Gregor 2007

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  1. Gregor 2007 • Use GFP-Bcd to further 2005 work • Use time-lapse two-photon microscopy • Fast and sensitive • See Bcd on nuclei and anterior to posterior gradient

  2. Gregor 2007 • Fluorescence increases with time

  3. Quick Summary • Bcd pattern seems to be established early and then stay regardless of • Nuclear proliferation • Nuclei size changes • Appearance of cell membranes • Rises and falls with mitosis • Stays constant within for different divisions • D is really small • Propose a new model that specifies nuclear degradation

  4. Castle • More recently have shown that characteristic lengths of source ~ gradient length • Suggest there might be localized production necessary to give rise to patterns • Big focus on methods  take issue with the photobleaching in Gregor 2007 • Model their work and find their estimate of D is off and diffusion model still holds

  5. Is GFP-Bcd functional/normal? • Yes. • GFP-Bcd can rescue a knockout • Pattern the same as wt • Stain for endogenous and GFP Bcd show colocalization

  6. Getting strange • Bcd associated with nuclei seems to spread out during mitosis? • Pretty constant

  7. Is it just binding/unbinding from nuclei? • Use photobleaching to show recovery • Implies transport across nuclear membrane • And also degredation • Make a new model

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