1 / 10

生物感測器原理與應用

生物感測器原理與應用. Surface Plasmon Resonance Biosensors. Surface Plasmon Resonance. --- SPR is a real-time, label-free, optical detection method for studying the interaction of soluble analyte with immobilized ligand --- completely non-specific

fox
Download Presentation

生物感測器原理與應用

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 生物感測器原理與應用 Surface Plasmon Resonance Biosensors

  2. Surface Plasmon Resonance --- SPR is a real-time, label-free, optical detection method for studying the interaction of soluble analyte with immobilized ligand --- completely non-specific 1902 Wood – anomalous diffraction due to excitation of surface plasma waves 1968 Otto – the method of attenuated total reflection 1988 (1990) BIAcore

  3. Total Internal Reflectance n1 > n2 evanescent distance ± 100 nm

  4. Thin Conducting Film A medium of high refractive index A thin layer with good electric conductivity A medium of low refractive index

  5. Characteristics of SPW Gold is the most practical. (In) too expansive, (Na) violently reactive, (Cu, Al) too broad in their SPR response (Ag) too susceptible to oxidation

  6. Configuration of SPR

  7. BIO-SUPLAR 2

  8. BIAcore

  9. Real-Time Kinetics

  10. Sensorgram

More Related