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Nitrate Reductase (NADH) EC 1.7.1.1

PRESENTASI ENZIMOLOGI KI- 5162. Nitrate Reductase (NADH) EC 1.7.1.1. Oleh : Dita Ayu Pratiwi (10506054) Sofi Siti Shofiyah (10506063). Agenda Presentasi. Tata nama enzim Kofaktor Mekanisme katalisis Manfaat enzim Struktur enzim Tahap isolasi dan pemurnian enzim

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Nitrate Reductase (NADH) EC 1.7.1.1

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  1. PRESENTASI ENZIMOLOGI KI- 5162 Nitrate Reductase (NADH)EC 1.7.1.1 Oleh: DitaAyuPratiwi (10506054) SofiSitiShofiyah (10506063)

  2. Agenda Presentasi • Tata namaenzim • Kofaktor • Mekanismekatalisis • Manfaatenzim • Strukturenzim • Tahapisolasidanpemurnianenzim • AplikasiIndustri

  3. IUBMB Enzyme NomenclatureEC 1.7.1.1 Sumber : • Springer Handbook of Enzymes

  4. KofaktorNitrate Reductase (NR)

  5. MekanismeKatalisis NR Sumber: Fischer, et. al. 2005 . The Plant Cell.

  6. Cleland Plot NR Sumber: Howard, et. al. 1981. Journal Biological Chemistry

  7. Asimilasi Nitrogen

  8. Stuktur 3D NR Sumber: Fischer, et. al. 2005 . The Plant Cell

  9. SisiAktifEnzimdanMuatanPermukaan NR Sumber: Fischer, et. al. 2005 . The Plant Cell

  10. Arabidopsis thaliana • Tanamanberbungakecil yang hidupdidataraneropa, asia, baratlautafrika • Tumbuhdimusim semi • Mempunyaisiklushidup yang relatifsingkat • Populersebagai model organismeuntukmemahamibiologimolekulbanyaktanaman

  11. ProduksiRekombinan NR Sumber: Wenpei Su, et. al. 1997. Plan Physiology.

  12. ProduksiRekombinan NR (Lanjt) Sumber: Wenpei Su, et. al. 1997. Plan Physiology.

  13. Permunian NR Sumber: Wenpei Su, et. al. 1997. Plan Physiology.

  14. Pemurnian NR (Lanjt) Sumber: Wenpei Su, et. al. 1997. Plan Physiology.

  15. PengujianKinetik NR Sumber: Wenpei Su, et. al. 1997. Plan Physiology.

  16. Aplikasi NR diIndustri • NR memilikiperanpentingdalambioteknologilingkungan. • Digunakansebagaimetodepengujiankomersialkandungannitratdalam air (detektorelektronikberbasiskanenzim yang akanmemonitoringsecarakontinukandungan nitrate dalam air) • Pengujianreduks nitrate

  17. Referensi Wenpei Su, Jeffrey A. Mertens, KengoKanamaru, Wilbur H. Campbell, and Nigel M. Crawford. 1997. Analysis of Wild-Type and Mutant Plant Nitrate Reductase Expressed in the Methylotrophic Yeast Pichapastoris’. Plant Physiology. William D. Howard4 and Larry P. Solomonsonf. 1981. Kinetic Mechanism of assimilatory NADH: Nitrate Reductase from Chlorella. Journal of Biological Chemistry Lawrie Skipper, Wilbur H. Campbell, Jeffrey A. Mertens, and David J. Lowe. 2001. Pre-steady-state Kinetic Analysis of Recombinant Arabidopsis NADH:NitrateReductase. JBC Papers. Katrin Fischer, Guillaume G. Barbier, Hans-Juergen Hecht, Ralf R. Mendel, Wilbur H. Campbell, and GuenterSchwarza. 2005 . Structural Basis of Eukaryotic Nitrate Reduction: Crystal Structures of the Nitrate Reductase Active Site. The Plant Cell. http://www.chem.qmul.ac.uk/iubmb/ http://www.plantphysiol.org/cgi/reprint/61/4/611 http://www.uniprot.org/uniprot/P11832

  18. Terimakasih Program Studi Kimia InstitutTeknologi Bandung 2009

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