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What Are You Drinking, TEA?

What Are You Drinking, TEA?. Tira J. Robinson Junior Biology Health Science Major Tennessee Technological University April 27, 2005. *Project Summary. To see what type of bacteria and e-coli grows in different teas To see how many coliforms grow from tea samples

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What Are You Drinking, TEA?

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  1. What Are You Drinking, TEA? Tira J. Robinson Junior Biology Health Science Major Tennessee Technological University April 27, 2005

  2. *Project Summary • To see what type of bacteria and e-coli grows in different teas • To see how many coliforms grow from tea samples • Take several different tea samples and run tests • Take a count of how many coliforms were present at the end of the experiment. • Give data and charts on results

  3. Introduction • There have been several media reports on the health hazards of fecal coliform contamination of restaurant brewed iced tea ( Doyle 1996). • In January the CDC issued an alert stating that ice tea can breed bacteria if it sits out all day (Kapner 1996). • There may be microbes, but they probably are of the relatively harmless variety that naturally cling to tea leaves (Nutrition 1996). • “The Centers for Disease Control and Prevention, which investigated the reports about iced tea, explained that theoretically tea could transmit disease if other bacteria survived the brewing (Iced 1996). • The large numbers of relatively benign bacteria that showed up probably occurred because of lax sanitation of restaurant tea dispensers (Nutrition 1996).

  4. Objective Statement • The objective for this experiment is to se what type of bacteria and microorganisms grow in different tea samples, if in they do, whether e-coli or metallic sheen is present.

  5. Hypothesis Statement • I hypothesize that microorganisms do grow in tea samples due to the tea leaves and or the amount of time tea is left sitting.

  6. Methods & Materials • Pour Plate method on TSA & Endo Agar Plates • Each plate will have a different measurement of .1, .5, 1 mL • Coliform count • Incubation of the plates for growth • Tea Samples from 3 different restaurants • Data will be recorded in tables • This experiment will be administered by Dr. Goss

  7. Results Endo Agar Plate Results • After incubation I found in restaurant A1 that it contained metallic sheen with fecal colifroms, which is e-coli. • All of restaurant A had grown mold • B1 also contained metallic sheen and fecal coliforms. • C1 only contained metallic sheen

  8. Results Cont. TSA plate results • All of the 3 restaurants contained fermentation • See Chart For More Details

  9. Data Endo Agar Plates Comments: A1 contained metallic sheen with fecal coliforms. A2 contained only fecal coliforms (e-coli). All of A had mold. B1 contained metallic sheen and fecal coliforms. C1 only contained metallic sheen. TSA Plates Comments: All restaurant samples contained fermentation

  10. Pictures

  11. Literature Cited • Doyle, Michael. Food Technology. Fecal conditions in tea: what’s the problem. Oct. 1996 v50 n10 p104(1) • Environmental Nutrition. Iced tea scare a tempest in a teapot. June 1996 v19 n6 p8(1) • Environmental Nutrition. Iced tea scare a tempest in a teapot. June 1996 v19 n6 p8(2) • Kapner, Suzanne. Iced-tea ‘brewhaha’: CDC says drink OK. June 1996 v30 n23 p1(2) • Marcinkowski, Victoria. Coffee, tea, or bacteria? Jan. 2003 v59 i8 p6(2)

  12. Conclusion • There are bacteria present in tea. • For the most part it isn’t harmful • Some of the causes can be from the tea leaves and or the time it has sat in high temperatures. • The growth of e-coli can be harmful • You can keep drinking tea!!!!!

  13. Acknowledgements • I would like to thank Dr. Goss for her time and help • A special thank you to Cara Sanders for an outstanding hand. • A special thanks to Kimberly Tuggle for being a great partner. • Thank you Dr. Morgan for all the help

  14. ANY QUESTIONS???? • Please Don’t ASK ANY!!!!!!!!

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