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Current Status of Chromosome II Sequencing

Current Status of Chromosome II Sequencing. Korea Research Institute of Bioscience and Biotechnology (KRIBB) Doil Choi. Organization and Coordination of the Tomato Chromosome 2 Sequencing Project. SGN Cornell. BAC FISH Dr. Jae-Wook Bang (CNU). PI Dr. Doil Choi (KRIBB). Sequencing

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Current Status of Chromosome II Sequencing

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  1. Current Status of Chromosome II Sequencing Korea Research Institute of Bioscience and Biotechnology (KRIBB) Doil Choi

  2. Organization and Coordination of the Tomato Chromosome 2 Sequencing Project SGN Cornell BAC FISH Dr. Jae-Wook Bang (CNU) PI Dr. Doil Choi (KRIBB) Sequencing Dr. Hong Seok Park (KRIBB) Informatics Dr. Cheol Goo Hur (KRIBB)

  3. Strategies for selecting seed BAC chr 2 BAC clone candidates marker sequence BAC DNA prep PCR primer HindIII enzyme cut Southern blotting overgo probe labeling PCR BAC FISH Hybridization choose seed BAC

  4. T0706 T1671 T0706 T1671 Problems on Selection of Seed BAC • Cross anchoring on other chromosome: 2 • No hybridization : 6 markers • Different size band : 4 markers cLEC-27-M9 T0123 cLEC-27-M9 T0123

  5. Result of Overgo Probing

  6. Result of BAC FISH

  7. Marker-anchored BACs selected by FISH cLED19B18 (28) cTOB9L18 (112) cLPT1A21 (114) cLEC-7-p21(0) cLEC27M9 (46) SSR104 (37) T1668 (37) T1480 (106) T0634 (130) T1554 (142) T1671 (82) T1438(70) T1096 (141) T1238 (13) T0869 (21) T1698 (34) T1654 (46) T1532 (69) T1537 (74) T0759 (82) T1202 (142) CT24 (136) T0683 (38) TG48 (92) TG373 (94) P61 (108) cLER1H17 (0) T1535 (88) T1555 (88) T1706 (18) SSR66 (25) 1 143 Chromosome II

  8. Current Summary of Chromosome 2 Sequencing

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