230 likes | 353 Views
Nasonia vitripenni. You are the PI ( P rimary I nvestigator). Jewel Wasp. Tell me about it…. Life Cycles. Haplo/Diplo Genetics. Wasp Development Timelines 18 o , 28 o C. Complementation. Process by which an allele of each of two mutant genes makes up for the defect in the other gene.
E N D
Nasonia vitripenni You are the PI (Primary Investigator)
Jewel Wasp Tell me about it…
Complementation Process by which an allele of each of two mutant genes makes up for the defect in the other gene.
You Find Three Mutants With a Similar Phenotype Are they the Same Genotype?
Possible Physiology Enzyme A Enzyme A Enzyme A Enzyme A Enzyme B Enzyme B Enzyme B Enzyme B Enzyme C Enzyme C Enzyme C Enzyme C
Complement a | | B a | | B A | | b A | | b x a | | B A | | b Two genes
Fail to Complement A | | b A | | b A | | b A | | b x A | | b A | | b 1 gene
Three scarlet eyed mutants, how many loci?stDR,stC,st318 stDR Connect same gene with a line. For example. Fail to complement st318 stC
Scoring Red Eyes Here
Wasp Business Link
To Do • Plan experiment! • define symbols, diagram crosses, determine expectations, etc. • Each student: complementation. • Per bench, or per pair (will tell tomorrow), • recessive, dominance, • controls (stock maintenance). Wednesday
Friday • Tinker with wasps, • aha3-1 segregation analysis. • Plant Arabidopsis in soil, • Introduction to Mitochondrial DNA, maternal genotyping, • Basic Mendal?