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Non-malignant Tumors samples

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Non-malignant Tumors samples

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  1. Supplementary Figure 1.Methylation level of 4 CpG sites located in the CYP24A1 promoter and gene in 33 nonmalignant lung samples and 153 lung AC included in the CURELUNG study. Non-malignant samples had a significantly higher β-value (p < 0.05, Wilcoxon rank sum test) for these CpG sites as compared to lung AC. cg02673418 cg02604290 cg24582168 cg24849555 p < 0.0001 p = 0.005 p = 0.0006 DNA methylation levels (β) p = 0.01 Non-malignant Tumors samples Non-malignant Tumors samples Non-malignant Tumors samples Non-malignant Tumors samples

  2. Supplementary Figure 2. (A) Quantitative RT-PCR of CYP24A1 expression in H460 and SK-LU-1 cells treated with increasing concentrations of 5-Aza followed by 100 nM of 1a,25-dihydroxyvitamin D3 (1,25-D3). (B) Quantitative RT-PCR of CYP24A1 expression in H460 and SK-LU-1 cells treated with increasing concentrations of TSA followed by 100 nM of 1,25-D3. Barplots and error bars represent mean and standard deviation of triplicate data points. (C) Basal protein expression of DNMT1 and DNMT3A in A549, NCI-H460 and SK-LU-1 cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. A B 5-Aza (mM) 1,25-D3 1,25-D3 C DNMT1 DNMT3A TSA (nM) GAPDH H460 A549 SKLU-1

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