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Seed sequence alignments annotated with secondary structure information obtained from Rfam and used for CM construction. Seed sequences for pHMM construction obtained from Rfam. Seed sequences for pHMM construction obtained from Rfam. BLAST (blastclust) .
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Seed sequence alignments annotated with secondary structure information obtained from Rfam and used for CM construction Seed sequences for pHMM construction obtained from Rfam Seed sequences for pHMM construction obtained from Rfam BLAST (blastclust) Non redundant seed sequences (<90% similar) Infernal (cmbuild) SAM (buildmodel) pHMM estimation from unaligned seed sequences Covariance models for the riboswitch family SAM(align2model) Alignment of seed sequences to pHMM Infernal (cmsearch) SAM(modelfromalign) pHMM for the riboswitch family HMMER(hmmsearch) Refseq database search Validation of the hits on the basis of genomic context