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Microbial Genetics (Micr340)

Microbial Genetics (Micr340) . Lecture 9 Lytic Bacteriophages (I). Definitions. Bacteriophages - viruses that infect bacteria (also called phages for short) Capsids - isocahedral heads of bacteriophages made of viral genome plus protein coats (for protection)

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Microbial Genetics (Micr340)

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  1. Microbial Genetics (Micr340) Lecture 9 Lytic Bacteriophages (I)

  2. Definitions • Bacteriophages - viruses that infect bacteria (also called phages for short) • Capsids - isocahedral heads of bacteriophages made of viral genome plus protein coats (for protection) • Plaques - holes (killing of bacterial cells by phages) on a lawn of bacterial growth

  3. Bacteriophage Lytic Cycle • Absorption to cell • Viral genome injected into cell • Viral gene transcription • Early genes • Middle genes • Late genes • Assembly of viral particles • Cell lyse

  4. Bacteriophage lytic cycle

  5. Regulation during lytic cycle

  6. Phage T4 • Large virus • Well studied • A new sigma factor • Replication-coupled transcription

  7. Phage T4: replication-coupled transcription

  8. Phage DNA replication • Single-stranded circular DNA • Linear DNA that forms concatemers

  9. Single-stranded circular DNA- phage f1

  10. Single-stranded circular DNAM13

  11. Single-stranded circular DNAM13

  12. M13 based cloning vector

  13. M13 based cloning vectorUse in site-specific Mutagenesis

  14. Linear DNA that forms concatemers –T7

  15. Linear DNA that forms concatemers –T4

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