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Adsorption Chromatography

Adsorption Chromatography. Adsorption Chromatography. Binding Polynomials, Q. Relative Population of Ligation States. Average number of ligands bound per P molecule, v=< i >. Average number of ligands bound per P molecule, v=< i >. Binding Polynomials. Binding Polynomials.

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Adsorption Chromatography

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  1. Adsorption Chromatography

  2. Adsorption Chromatography

  3. Binding Polynomials, Q

  4. Relative Population of Ligation States

  5. Average number of ligands bound per P molecule, v=<i>

  6. Average number of ligands bound per P molecule, v=<i>

  7. Binding Polynomials

  8. Binding Polynomials

  9. Two Binding Sites: Cooperativity • Average number of ligands per P molecule,

  10. Two Binding Sites: Cooperativity • Independent sites: Non-cooperative binding • Not independent sites: • Cooperative binding • Anti-cooperative binding

  11. Two Binding Sites: Stoichiometric Approach

  12. Two Binding Sites in Glycine

  13. Two Binding Sites: Stoichiometric Approach

  14. Two Binding Sites: Stoichiometric Approach

  15. Ligation States for Two Binding Sites Relative Population of 0 ligandbound: 1/Q Relative Population of 1 ligand bound: K1x/Q Relative Population of 2 ligand bound: K1K2x2 /Q

  16. Site-model and stoichiometric model binding constants

  17. n Independent Sites with Identical Affinities

  18. n Independent Sites with Identical AffinitiesScatchard Plot

  19. n Independent Sites with Identical AffinitiesScatchard Plot

  20. ScatchardPlot for an Antibody

  21. Cooperative Binding

  22. Cooperative BindingThe Hill Plot

  23. Cooperative BindingThe Hill Plot

  24. Cooperative BindingThe Hill Plot

  25. Cooperative BindingThe Hill Plot

  26. Aggregation and Micellization are Cooperative Processes

  27. Critical Micelle Concentration, cmc

  28. Micelle Well-Defined Sizes

  29. The BET Adsorption Model of Layers of Ligands

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