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課程名稱： 論文選讀（上） 學分數： 1 開課系所： 生科四（甲） 上課時間： Tue,

課程名稱： 論文選讀（上） 學分數： 1 開課系所： 生科四（甲） 上課時間： Tue, 任課教師： 賴金美老師 Office : LS303, 分機： 3595. * 需 撰寫摘要 並附上論文圖表及說明；摘要需於前一週前交給老師以 做修改，並於報告當天發給每位同學。 * 口頭報告 40~45 分鐘，同學或老師提問 15~20 分鐘。 * 由前一次同學負責借用及歸還投影機並擔任該週報告之主持人（負 責介紹及發問）。 評分： Presentation: 60%

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課程名稱： 論文選讀（上） 學分數： 1 開課系所： 生科四（甲） 上課時間： Tue,

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  1. 課程名稱： 論文選讀（上） 學分數： 1 開課系所： 生科四（甲） 上課時間：Tue, 任課教師： 賴金美老師 Office : LS303, 分機：3595

  2. * 需撰寫摘要並附上論文圖表及說明；摘要需於前一週前交給老師以 做修改，並於報告當天發給每位同學。 * 口頭報告40~45分鐘，同學或老師提問15~20分鐘。 * 由前一次同學負責借用及歸還投影機並擔任該週報告之主持人（負 責介紹及發問）。 評分： Presentation: 60% [Abstract (20%); Presentation & Discussion (40%)] Raising question: 15% Final report (modified power point file): 15% 出席率：10%（遲到扣分：0.5分/次） If you still have questions, please do not hesitate to contact me by bio2028@mails.fju.edu.tw

  3. The mechanism of RNAi ~ the expression of ds RNA induces the specific degradation of the mRNA to which it is complementary. * siRNAs: small inhibiting RNAs * Dicer: ds-specific RNase enzyme (21-25 nts) * RISC:RNA induced silencing complex ~ degrade the mRNA-siRNA duplexes.

  4. Traditional RNAs

  5. Central Dogma of Molecular Biology Genome Small non-coding RNAs play an important role in gene regulation. rRNA tRNA miRNA siRNA mRNA Protein Cellular responses

  6. RNA Types RNA mRNA Protein-coding RNA ncRNA Non-coding RNA. Transcribed RNA with a structural, functional or catalytic role tRNA Transfer RNA Interface between mRNA & amino acids snRNA Small nuclear RNA Incl. RNA that form part of the spliceosome snoRNA Small nucleolar RNA Found in nucleolus, involved in modification of rRNA rRNA Ribosomal RNA Participate in protein synthesis RNAi RNA interference Small non-coding RNA involved in regulation of expression Other Including large RNA with roles in chromatin structure and imprinting siRNA Small interfering RNA Active molecules in RNA interference miRNA MicroRNA Small RNA involved in regulation of expression Modified from GENES VIII, p. 301-324, 703.

  7. Discovery of RNA Interference • In 1990 , Napoli C. et al. trying to deepen the purple color of petunias, introduced a pigment-producing gene under the control of a powerful promoter. Instead of the expected deep purple color, many of the flowers appeared variegated or even white. • Napoli C. et al. named the observed phenomenon "co-suppression" • PTGS(post- transcriptional gene silencing) The Plant Cell, 2, 279-289, 1990

  8. Discovery of RNA Interference • In 1995, Su Guo found that the "sense" nucleic acids they used as controls, which shouldn't bind to the active genes or mRNAs, proved just as effective as the antisense constructs in blocking gene (par-1) expression of Caenorhabditis elegans. • In1998, Andrew Fire and Craig Mello found the double-stranded RNA was much more effective than either the sense or antisense RNA, they named the observed phenomenon “RNA interference”. Craig Mello Su Guo Andrew Fire

  9. RNAi

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