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REPRESSILATOR

REPRESSILATOR. SILAS FERRAO. WHAT IS IT?. IN 2000 STANISLAS LIEBLER AND MICHAEL ELOWITZ CREATED GENETIC MACHINE CALLED REPRESSILATOR-MILESTONE IN SYNTHETIC BIOLOGY, IT WAS AN OSCILLATORY GENTIC MACHINE USED E. COLI TO EXHIBIT REGULATORY NETWORK. HOW DOES IT WORK?. “GENETIC CLOCK”

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REPRESSILATOR

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  1. REPRESSILATOR SILAS FERRAO

  2. WHAT IS IT? • IN 2000 STANISLAS LIEBLER AND MICHAEL ELOWITZ CREATED GENETIC MACHINE • CALLED REPRESSILATOR-MILESTONE IN SYNTHETIC BIOLOGY, IT WAS AN OSCILLATORY GENTIC MACHINE • USED E. COLI TO EXHIBIT REGULATORY NETWORK

  3. HOW DOES IT WORK? • “GENETIC CLOCK” • TETR, LACL, λCL ARE REPRESSORS • PTET, PLAC, λPR ARE PROMOTERS • ARRANGED ON PLASMID ALONGSIDE GFP PLASMID

  4. HOW DOES IT WORK? LACL L-PR

  5. PROBLEMS….. • OSCILLATIONS LAST FOR TWO AND A HALF HOURS • LOSE SYNCH WITHIN SYSTEM • SYSTEM IS “NOISY”

  6. DEVICE…. CELIAC DISEASE I HATE IT WHEN THIS HAPPENS TO ME…

  7. WHAT IS IT? • AUTOIMMUNE DISORDER IN SMALL INTESTINE • DAMAGES THE VILLI (ATROPHY) • REACTION TO GLIADIN

  8. GLIADIN • GROUP OF PROTEIN FOUND IN GLUTEN • A-GLIADIN • GLUTEN HALF OF THE WHEAT SEED

  9. DEVICE Cyclic AMP CR CXCR3 R2-HMW Chromosome GLN BP Plasmid H. PYLORI RHO TERM. HD RBS E. COLI ORF A-gliadin promoter

  10. HD RBS

  11. RESULTS…. • VERY IMPRACTICAL • GLUTEN ANGLE • RECEPTORS, TERMINATORS, BACTERIA ONLY TO DEAL WITH INHBITING GLUTEN CROSSLINKING

  12. THANK YOU DOE!

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