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GMO Investigator Kit Is your food genetically modified?

GMO Investigator Kit Is your food genetically modified?. Philander Smith College – BIO204 Genetics. Nastassia n . jones. Why teach GMO testing?. Kit Controls. Why use CaMV 35S and NOS ?. CaMV 35S – Sequence for the promoter of 35S transcript of the Ca uliflower m osaic v irus.

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GMO Investigator Kit Is your food genetically modified?

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  1. GMO Investigator KitIs your food genetically modified?

  2. Philander Smith College – BIO204 Genetics Nastassia n. jones

  3. Whyteach GMO testing?

  4. Kit Controls

  5. Why use CaMV 35S and NOS? CaMV 35S – Sequence for the promoter of 35S transcript of the Cauliflower mosaic virus. Used because it functions in every plant cell NOS- Sequence fornopalinesynthase terminator from soil bacterium Agrobacteriumtumefacians Used because it evolved to be recognized in most plants

  6. Method for Genetic Modification of Crops • Choose desirable trait • Clone the gene • Engineer the gene • Transform gene into plant • Backcross GM plant into high yield crops

  7. Choose desirable trait • Pest Resistance: Bt crops • Bacillus thuringiensis protein is a delta endotoxin kills corn borers • HerbicideTolerance: Round Up Ready crops • Agrobacteriumtumifaciens protein with resistance to Round Up herbicide (glyphosate) Bacillus thuringiensis Delta endotoxin crystal

  8. GMO Investigator KitLab Extensions • Independent studies • Data Mining/Bioinformatics for specific genes • E.g. Design primers to the cry genes in Bt corn • Quantitative Real-Time PCR

  9. EPSP synthase

  10. EPSP synthase catalyzes the reaction which converts shikimate-3-phosphate plus phosphoenolpyruvate to 5-enolpyruvylshikimate-3-phosphate (EPSP).

  11. NCBI • Search for EPSP synthasea.a. sequence in Agrobacterium • BLAST a.a. sequence to find in other organisms • NCBI • IMG • InterPro (find out more info about protein) • Multiple Sequence alignment

  12. InterPro

  13. Pfam

  14. Pfam-alignments

  15. HMM logo

  16. Protein Sequence Search

  17. Protein Structure

  18. ConSurf

  19. Thank you!

  20. BioNJ

  21. TNT

  22. PhyML

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