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PTP catalytic mechanism

PTP catalytic mechanism. the general acid. the general base. WPD loop. D to A mutant. DA (trapping) PTPs. WT (active) PTPs. PTP1B +/+. PTP1B -/-. Background PTP1B Knock Out Mice. PTP1B -/- mice display enhanced sensitivity to insulin

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PTP catalytic mechanism

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  1. PTP catalytic mechanism the general acid the general base

  2. WPD loop D to A mutant

  3. DA (trapping) PTPs WT (active) PTPs

  4. PTP1B +/+ PTP1B -/- Background PTP1B Knock Out Mice PTP1B -/- mice display enhanced sensitivity to insulin PTP1B -/- mice are resistant to development of obesity and Type 2 diabetes (Elchebly et al. Science (1999) 83, 1544-1548)(Klaman et al. Mol Cell Biol (2000) 20, 5479-89)

  5. PTP Inhibition of the Insulin Receptor PTP may Enhance Insulin Signaling Prolonged or Enhanced Signal Transient Signal

  6. Structure of the PTP1B-IR peptide complex G1166 R1164 Phe182 Arg24 Arg254 pY1163 pY1162 Asp48 D1161 Tyr46 Arg47

  7. IRTK Activation segment: DIpY1158ETDpY1162pY1163RKGG S.R. Hubbard, (1997) EMBO J., 16, 5573-5581

  8. Kinetic Constants for Dephosphorylation of IR Activation Loop Peptide by PTP1B RDIpY1158ETDpY1162pY1163RKGGKGLL

  9. Insulin Insulin Receptor PTK PTP1B Signaling Response PTP1B and insulin signaling

  10. PTP Suppression of PTP1B expression by RNAi may enhance tyrosine phosphorylation of IR 972 1158 1162 1163 Prolonged or Enhanced Signal Transient Signal

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