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  1. Figure 2. Transfection efficiency in siRNA transfected NICC. NICC were transfected with the BLOCK-iT™ Fluorescent Oligo (FITC-conjugated) and transfected NICC were identified as FITC-positive cells. Data represent one of five independent experiments and mean fluorescence intensity of FITC positive cells is presented as mean ± SEM. Open histogram represents non-transfected NICC and filled histogram represents NICC transfected with the BLOCK-iT™ Fluorescent Oligo 64 MFI = 3.3  0.3 Cell counts 69.2%  8.7% MFI = 35.5  2.0 0 100 101 102 103 104 Fluorescence intensity Supplemental Digital Content 2

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