1 / 35

Ahmed El-Sohemy

Coffee, Caffeine and Cardiovascular Disease. Ahmed El-Sohemy. Canada Research Chair in Nutrigenomics. Department of Nutritional Sciences University of Toronto. Nutrigenomics:. Using genomic information and techniques in molecular genetics & genomics to address

garren
Download Presentation

Ahmed El-Sohemy

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Coffee, Caffeine and Cardiovascular Disease Ahmed El-Sohemy Canada Research Chair in Nutrigenomics Department of Nutritional Sciences University of Toronto

  2. Nutrigenomics: Using genomic information and techniques in molecular genetics & genomics to address issues important to nutrition and health. Diet Genes Food preferences

  3. Why bother with genetics/genomics?  Challenges for Nutritional Epidemiology Measurement error Confounding (residual) Recall Bias (case-control)

  4. Is Coffee associated with CVD?  Risk No Effect  Risk

  5. Is Coffee associated with CVD? • Coffee or associated lifestyle? • Are coffee abstainers at  risk? • Does risk depend on age? • Which component of coffee?

  6. Bioactives in coffee aliphatic acids magnesium potassium caffeine caffeine melanoidins diterpenoids polyphenols

  7. Caffeine (1,3,7-trimethylxanthine)

  8. SNPs SNPs Caffeine Metabolism Caffeine Target CVD Coffee

  9. SNPs SNPs Caffeine Metabolism Caffeine Target CVD Coffee

  10. Caffeine 1,7-dimethyluric acid 5-acetylamino-6-formylamino-3-methyluracil 1-methylxanthine 1-methyluric acid CYP1A2 Paraxanthine

  11. Genetic Variation in CYP1A2 -163 AC fast slow

  12. Costa Rica Study • 2013 cases (myocardial infarction) • 2013 population-based controls - matched (age, sex, area of residence) • Data collection: - food frequency questionnaire - health and lifestyle questionnaire - fasting blood sample (DNA)

  13. CYP1A2 Genotype Distribution rapid slow

  14. Coffee Intake and Risk of Myocardial Infarction * *P<0.05 Cornelis et al., JAMA 295: 1135-41, 2006

  15. Coffee Intake and Risk of Myocardial Infarction * * A/A A/C + C/C *P<0.05 Cornelis et al., JAMA 295: 1135-41, 2006

  16. Coffee Intake and Risk of Myocardial Infarction Subjects <50 Years of Age * * * * A/A A/C + C/C *P<0.05 Cornelis et al., JAMA 295: 1135-41, 2006

  17. Personalized Dietary Advice vs Public Health Recommendations

  18. Recommended Maximum Caffeine Intake (Health Canada)

  19. Genetic modifiers of exposure Genetic modifiers of biological effect CYP1A2 FMO CYP2A6 NAT2 CYP2E1 UGT SULT CYP1A2 CYP3A4 CYP2D6 CYP1A1 UGT SULT CYP2C9 CYP2E1 UGT GST SULT ADR ADORA HTT GRK HTR COMT CETP PLTP LDLR ACAT SREBP GST ACE TNF NFkB VCAM1 PKC PPARG PDE CREB GLUT PKA GLP1R GLUT FAS1 ACE NOX G6PT1 Caffeine Diterpenes CHD Risk Flavonoids Polyphenols Curr. Opin. Lipidol, 18: 13-19, 2007

  20. Health Outcome Coffee Genotype A Increase Genotype B No effect Genes Genotype C Decrease

  21. Caffeine Consumption Habits Study • Healthy Men and Women aged 20-29 years (n=940) • Food Frequency Questionnaire • Caffeine consumption • Caffeine Consumption Habits Questionnaire • - Acute effects of caffeine • - Caffeine withdrawal symptoms • - Limiting or avoiding caffeine

  22. DRD2 C957T Genotype and “Elevated Mood” Among Men and Women ** * *P<0.05 **P<0.005

  23. DRD2 C957T Genotype and Caffeine Intake Among Men and Women * *P<0.05

  24. ACKNOWLEDGMENTS Hannia Campos Edmond Kabagambe Tom Wolever CCD Investigators Paul Corey David Jenkins Hyeon-Joo Lee Lindsay Stewart Daiva Nielsen Alejandra Navarro-Allende Susana Huang Sarah Herd Darren Brenner Bibiana Garcia-Bailo Marilyn Cornelis Leah Cahill Karen Eny Bénédicte Fontaine-Bisson Ilana Platt Stephen Ozsungur Clare Toguri Advanced Foods and Materials Network Centres of Excellence Natural Sciences and Engineering Research Council Canadian Institutes of Health Research Canada Research Chairs

More Related