1 / 8

Biotechniques

Biotechniques. Tissue Culture (micro-propagation). A method of rapidly cloning desired plants [few weeks]. Plant cells are totipotent (can grow into whole plant) Process is repeated to generate millions of plants from one explant. Conditions.

gavril
Download Presentation

Biotechniques

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Biotechniques

  2. Tissue Culture (micro-propagation) Amethod of rapidly cloning desired plants [few weeks]. Plant cells are totipotent(can grow into whole plant) Process is repeated to generate millions of plants from one explant.

  3. Conditions Sterile (avoid fungal and bacterial growth) essential food, mineral and vitamins auxins and cytokinins

  4. Method small explant is cut and sterilised explant placed on nutrient agar medium transferred to growing medium grows into callus (mass of undifferentiated cells) hormones cause cell differentiation  new plants planted out and hardened off

  5. Advantages of tissue culture copy desirable plants virus free explants prone to virus infection (can study effects of infection) cultures subjected to stress – select cells that survive (hardy) for cloning can transfer new genes into cell (with a DNA gun) prior to cloning

  6. Tissue culture http://www.youtube.com/watch?v=kje0YczE0Do (tissue culture, 7 min) http://www.dnatube.com/video/1008/Tissue-Culture (a bit boring, but not youtube, 7 min, sound and video way out of synch and cut off half way through?)

More Related