10 likes | 98 Views
A. V. Virus. 1 h 1 h 18 - 19 h 45 min each step. Fluorescence staining. I. Inhibitor. Anti-nucleoprotein monoclonal Ab conjugated mouse IgG. + MgCl 2. Treatment. Viral adsorption. Ex336/Em490. Pretreatment. β-galactosidase labeled anti-mouse IgG. 4-Methylumbelliferyl galactoside.
E N D
A V Virus 1h 1h18 - 19h45mineach step Fluorescencestaining I Inhibitor Anti-nucleoprotein monoclonal Ab conjugated mouse IgG + MgCl2 Treatment Viral adsorption Ex336/Em490 Pretreatment β-galactosidase labeled anti-mouse IgG 4-Methylumbelliferyl galactoside V+I I 4-Methylumbelliferyl products MDCK Oseltamivir carboxylate, mM B PVP-I, mg/ml MUーGAL MUーGAL Relative inhibition, % Relative virus yield, % MU MU A/Bel/42 A/WS/33 A/Aichi/2/68 A/PR/8/34 A/WSN/33 A/DK/HK/313/78 A/DK/HK/36/4 A/Memphis/1/71 A/DK/HK/677/1 A/DK/HK/313/78 A/USSR/92/77 A/DK/HK/23/76 A/Memphis/1/71 A/DK/HK/92/76 A/Texas/36/91 HumanAvianHumanAvianAvian Human Avian H1N1H3N2H5N3H9N2H3N2H5N3