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Automating SPE Extractions with the HT400E System

Automating SPE Extractions with the HT400E System. by William Amoyal Disruptive Technologies. Disruptive Technologies history. Distribution company founded in 2005 Based in Villecresnes, France Cover France, Belgium, Switzerland and other countries depending on product range

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Automating SPE Extractions with the HT400E System

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  1. Automating SPE Extractions with the HT400E System by William Amoyal Disruptive Technologies

  2. Disruptive Technologies history Distribution company founded in 2005 Based in Villecresnes, France Cover France, Belgium, Switzerland and other countries depending on product range Agilent Service Partner for UV/Vis spectrophotometry, HPLC and CE 2 people William Amoyal, Sales Manager Franck Do Vale, Service Manager

  3. Sample Preparation Nucleic acides, protein and small molecules extraction from hard-to-lyse tissue samples Online SPE system (couples with LCMS) Dissolution/Formulation Dissolution baths, friability and disintegration instruments Physico Chemistry HT Log P and pKa analyzer HT oligonucleotides purity analyzer HT proteins analyzer HT DNA analyzer HT Chiral analyzer Kinetic solubility instruments Service Agilent Channel Partner to service their range of HPLC, UV Spectroscopy and CE Rapid microbiology with Micro PRO Analytical/preparative OPLC chromatography solutions for semi preparative applications (OPLC systems, pumps, sample applicator, video imaging and densitometry instruments, reagent sprayer. Spotter for MALDI and tissue MALDI imaging ELSD detectors Type-C silica hydride HPLC columns Flat sorbent beds for OPLC Accessories and consumables for CE and HPLC Validation kits for HPLC systems Disruptive Technologies product range

  4. Benefits of HT400E system • Reliable • Based on reliable technology of HTA autosampler already used by Agilent, Shimadzu, Perkin etc ... In their GC systems • Reproducible • Very low CV% inter-assay <2,5% • Flexible • Accepts many types of SPE cartridge, sample and target vials • User friendly • Intuitive “drag-and-drop” software • Competitive Price • Highest cost-to-performance SPE system available on the market

  5. HT400E system with cooling unit

  6. Main features of HT400E system • Can be used as an SPE system or HPLC autosampler • 20/29 samples max per tray • 4 solvents available (more on request – max 11) • Syringe and needle washing • Software manager QUICK-PREP • Sample tray Peltier cooling unit (optional)

  7. Sample tray configurations • 20/29 samples per TRAY • Sample volume: ∞ ml • SPE • 1ml , 3ml standard • 6 ml optional • Target Volume: • 2 ml • 4 ml • Syringe: • 1 ml • 2.5 ml • Other on request

  8. Ideal workflow • Cartridge activation • Volume, n. of solvent, n. of cycles • Cartridge drying • # of cycles • Sample loading • Volume, # of cycles • Sample washing • Volume, # of solvent, # of cycles • Elution • Volume, # of solvent, # of cycles, different target vials • Reagent addition • Volume, # of reagent, different target vials • Washing syringe and needle • # of cycles • Post-washing cartridge to re-use it

  9. Easy-to-use Quick-Prep software • Drag-and-drop software • Tools such as Draw, Dispense, Mixing, Elution, Drying, Cartridge Picking, Cartridge Carry, Injection, Valve Washing, Cartridge Conditioning etc ... can be dragged and dropped in method making it possible to write methods in minutes • Easy and intuitive software • Easy set-up of the workstation (WorkTable) • Easy-write work-up process (WorkFlow) • Possibility to link methods

  10. Application examples and results • The following evaluation has been made using the VMA kit of Chromsystems • The procedure has been as requested by Chromsystems protocol

  11. Chromatogramme of VMA separation

  12. Reproducibility of VMA separation

  13. Carry-over on pathological control Pathological Control

  14. Carry-over on water WATER

  15. Specifications

  16. For more information on HT400E system • To see a video, click on picture besides in Diaporama mode or copy following URL in your browser http://www.youtube.com/watch?v=XApeb60kGUs • To download a brochure click on or copy following URL HT400E SPE system brochure http://wamoyal.free.fr/LabHut/SPE/HT400E%20SPE%20system.pdf

  17. Contact details at Disruptive Technologies Contact William Amoyal, Sales Manager Disruptive Technologies 3 Allée des Camélias 94440 Villecresnes Mobile: +33 (0)6 98 64 98 81 Email: wamoyal@disruptechno.com Web: www.disruptechno.com

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