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Review for Photosynthesis Test

Review for Photosynthesis Test. -Lab you did today -Lab on Lab Bench (we are going to run thru it now) -Material from Photosynthesis Lecture. Photosynthesis Virtual Lab. What exactly did we measure again? CO2 Output=net photosynthetic rate= CO2 Consumed (P) – CO2 produced (R)

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Review for Photosynthesis Test

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  1. Review for Photosynthesis Test -Lab you did today -Lab on Lab Bench (we are going to run thru it now) -Material from Photosynthesis Lecture

  2. Photosynthesis Virtual Lab • What exactly did we measure again? CO2 Output=net photosynthetic rate= CO2 Consumed (P) – CO2 produced (R) -How did temperature affect this rate? -How did CO2 affect this rate? -How did light intensity affect this rate?

  3. Lab on Lab Bench: 2 Parts • Chromatology= this separates plant pigments based on their molecular weights and hydrogen bonding with paper.

  4. Rf (reference front) value= distance pigment moved/distance solvent migrated. So if the solvent migrated 10 cm… 6 CM 4 CM 2 CM

  5. Quiz 1 on Lab Bench asks 3 questions about this…be sure you can do this. • Now for the second part of lab bench…this is the DPIP part. • DPIP is an electron acceptor. We use this chemical because it turns from blue to clear as it accepts excited electrons during photosynthesis. • We use a Spectrometer to measure this color change. (No spec )

  6. Transmittance % TIME

  7. “Design an Experiment” • 1. Use DPIP as electron acceptor. It changes from blueclear as it is reduced. • 2. Measure the change in light transmittance with a spec. • 3. Include a blank with no DPIP to calibrate Spec. • 4. Include control. Include experimental treatments. • 5. Measure transmittance and graph results.

  8. Photosynthesis Review • What are the four different pigments? • What are the three parts to a photosystem? Antenna complex Reaction center chlorophyll Primary Electron Acceptor

  9. Photosystem I=has specialized chlorophyll a molecule known as p700 • Photosystem II= has specialized chlorophyll a molecule known as P680 • Photosystem 2 Photosystem 1 (Noncyclic) • Photosystem1= (Cyclic)

  10. Calvin Cycle • Make sure you know the input and output of this cycle (know the numbers) • Words to know such as: rubisco, PGA, PGAL • 6CO2+ 18 ATP + 12 NADPH +H  18 ADP + 18 Pi + 12 NADP+ 1 gulcose

  11. C4…the new way! • PEP vs. Rubisco • OAA, malate CAM Photosynthesis…OAA malic acid gets sent to the vacuole! -same as C4 except malic acid instead of malate What is photorespiration?

  12. 1. Oxygen is released here. • 2. Stores energy obtained from light into NADPH. • 3. Occurs in the stroma. • 4. How is C4 different from C3? • C4 plants require less water • C4 plants can photosynthesis better in lower levels of light • C4 plants are more efficient CO2 fixers

  13. 1. An outcome in noncyclic vs. cyclic outcomes? • 2. Colors of all of the pigments? • 3. How does photosynthesis compare to respiration? Essay Section: • One essay will want you to describe the whole photosynthesis process. • Other essay will be about photosynthesis labs (lab bench + what we did in class).

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