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Relationship of Gene’s Type and Introns

Relationship of Gene’s Type and Introns. Chi K. To. Eukaryotes contain multiple introns per gene. Throughout eukaryotic evolution, the length of introns had been changed by either insertion, deletion or both. How big of these lengths had been change?

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Relationship of Gene’s Type and Introns

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  1. Relationship of Gene’s Type and Introns Chi K. To

  2. Eukaryotes contain multiple introns per gene. • Throughout eukaryotic evolution, the length of introns had been changed by either insertion, deletion or both. • How big of these lengths had been change? • Do they just happen in specific type of genes? What are those types?

  3. Method: • Use the Browser to get introns in the whole genome. • Do pairwise alignment between human and dog genomic sequences with the reconstructed sequences of their common ancestor. • Only select introns have gaps that are greater than 100bp.

  4. Insertion (gaps on ancestral sequences) , deletion (gaps on human/dog sequences). • Get genes’ IDs • Upload data to the http://gostat.wehi.edu.au/ - help to calculate the Hypergeometric distribution for the data • Gene ontology classification of introns.

  5. Result: Insertion in Human

  6. Deletion in Human

  7. Insertion in Dog

  8. Deletion in Dog

  9. * In human: Genes have the molecular function related to GTPase regulator activity or locate in plasma membrane tend to change the lengths of introns within the genes during the evolutionary process. * In Dog: Genes have the biological process related to Actin cytoskeleton organization and biogenesis and the mol. munction related to small GTPase regulatory/interacting protein activity tend to change the lengths of introns within the genes during the evolution.

  10. A big deletion of a intron in gene NM_018283 of Dog

  11. Special thanks to: 1/ Baertsch, Robert – pairwise alignment data 2/ http://gostat.wehi.edu.au/ - for the G.O. statistics 3/ UCSC genome Browser

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