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T reatments that stimulate the E.coli . to take up foreign plasmids include:

T reatments that stimulate the E.coli . to take up foreign plasmids include:. CaCL2 treatment Heat shock Incubation with ECORI 1 and 2 2 and 3 All of the above. If transformation with the Pglo plasmid is successful, growth should occur on all plates except:. +DNA/lb/amp/ ara

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T reatments that stimulate the E.coli . to take up foreign plasmids include:

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  1. Treatments that stimulate the E.coli. to take up foreign plasmids include: • CaCL2 treatment • Heat shock • Incubation with ECORI • 1 and 2 • 2 and 3 • All of the above

  2. If transformation with the Pglo plasmid is successful, growth should occur on all plates except: • +DNA/lb/amp/ara • +DNA/lb/amp • -DNA/lb/amp • -DNA/lb

  3. In the green fluorescent colonies, what is fluorescing? • The Pglo plasmid • The GFP • Arabinose • Uv light

  4. What is the puropose of the arabinose in the Pglo transformation lab? • It make the E. coli. competent • It helpd the E.coli take up the plasmid • It turns on the GFP gene • It turns on the amp resistance gene • 3 and 4

  5. In what region of the lambda virus genome is gene H found? • Head proteins • Tail proteins • Attachment/integration/excision • Control region • lysis

  6. What is ECORI used for: • Making gels • Making E.coli competent • Digesting DNA • Turning on the H gene

  7. A gene found on the PUC-18 plasmid not found on the Pglo plasmid: • Bla • Lac Z • ECORI • 2 and 3

  8. How many DNA fragments are produced why lambda DNA is digested with ECORI? • 1 • 2 • 4 • 6 • 7

  9. After colonies grow on the Xgal/amp agar plate, what do you do next with the colonies? • Transform them • Treat them with ECORI • Heat shock them • Treat them with CaCl2 • Transfer them to a tube of broth

  10. Lysozyme is used in: • Electrophoresis • Trasnformation • Plasmid isolation • Making recombinant PUC-18 plasmids

  11. What is agarose used for? • Making gels • Making E.coli competent • Digesting DNA • 4. Turning on the H gene

  12. What does electrophoresis sample buffer contain? • ECO RI • CaCL2 • Tracking dye • Glycerol • 2 and 3 • 3 and 4

  13. The wells of the electrophoresis are placed nearest: • The negative terminal of the chamber • The positive terminal of the chamber

  14. There are 4 DNA markers that we applied to our gel (784, 1120, 2010 and 3621). Which of these will migrate the furthest away from the wells during electrophoresis? • 784 • 1120 • 2010 • 3621

  15. In which ECORI fragment of lambda DNA do we expect to find the H gene? • 3530 • 4878 • 5804 • 5643 • 7421 • 21226

  16. Which colonies contain the plasmids that have a PUC-18 plasmid with lambda DNA inserted into them ? • Blue colonies • White colonies • Green fluorescent colonies

  17. In order to determine which lambda DNA fragment was inserted into cells from white colonies, which technique(s) did you do in lab? • Prepare recombinant PUC-18 /lambda DNA plasmids • Plasmid isolation • Electrophoresis • ECO RI digestion of recombinant PUC-18 /lambda DNA plasmids • 3 and 4

  18. Which of the following is the lambda genomic library? • The tube of recombinant PUC-18/lambda DNA plasmids • The stained electrophoresis gel • The Xgal/amp agar plate with white colonies • The broth tube of a white colony from the X-gal/amp agar plate.

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