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BC and Me – 1980 to 2003

BC and Me – 1980 to 2003. 1980: First Meeting –NMR meeting 1984: Joined Brit’s lab 1984-87: Animal Models Open Chest Animals: dog and cat Volume & pressure overload; ischemia; hypoxia Closed Chest animals: dog Volume & pressure overload; cardiomyopathy; heart failure; ischemia

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BC and Me – 1980 to 2003

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  1. BC and Me – 1980 to 2003 • 1980: First Meeting –NMR meeting • 1984: Joined Brit’s lab • 1984-87: Animal Models • Open Chest Animals: dog and cat • Volume & pressure overload; ischemia; hypoxia • Closed Chest animals: dog • Volume & pressure overload; cardiomyopathy; heart failure; ischemia • Other intact organs: Skeletal Muscle; liver, brain • Increase work load; ischemia • 1987-2003: Isolated organs, cells & mitochondria

  2. Multiple Technologies • NMR: 31P, 23Na; 1H; 13C; 19F • NADH Fluorescence • NIR Spectroscopy • Other physiological measurements • Piezoelectric crystals: Mechanical function • Laser Doppler: flow in blood vessels; tissue perfusion • Tissue electrodes: Cae2+, Ke+, Nae+ • ECG, EEG • BP, HR, LVP

  3. World Wide Colleagues • British Connection: John Haselgrove • Chinese Connection: Daning Zhang; DJ Wang • French Connection: Bertrand Autlier; Suzanne Wehrli • German Connection: Manfred Prammer • Hungarian Connection: Laci Ligeti; Tomas Ivanics; Aristied Kovach: Kati Megyeri; Laci Deszi • Indian Connection: Hari Subramanian; Ramin Mitra • Israeli Connection: Avraham Mayevsky; Myer Shinnar • Japanese Connection: Shoko Nioka; K Niibori • Netherland Connection: Hans van Beek • Polish Connection: Maria Erecinska; Krzysztof Wroblewski • Russian Connection: Andre Savchenko • Ukrainian Connection: Nicola Doliba; Natalya Doliba; Andriy Babsky • Uruguayan Connection: Juan Crestanello • US Connection: too numerous to list • The World: too numerous to list

  4. NMR Spectroscopy – Multiple collaborations • Intact in vivo brain • Closed Chest Animals: Dog; Cat • Open Chest animals: Dog; Cat • Perfused Heart • Superfused Cardiomyocytes; hepatocytes • Superfused Mitochondria – heart, liver

  5. Closed Skull Cat Brain

  6. Closed Skull Cat Brain – 31P

  7. Open Chest Animal: Dog or Cat

  8. Open Chest Dog – 31P

  9. Closed Chest Dog – 31P

  10. Langendorff Perfused In NMR

  11. Isolated Perfused heart – 31P

  12. Superfusion SystemIsolated Cells & Mitochondria

  13. Isolated Superfused Cardiomyocytes -31P

  14. Isolated Superfused Mitochondria- 31P

  15. Optical Spectroscopy&Electrode System • NADH Fluorescence • Heart • Brain • NIR • Oxygenation • Multiple electrode system • Ca2+ • K+ • Na+

  16. NADH Fluorescence & NMR

  17. NADH FluorescenceHypoxia & NE Administration

  18. Multiparameter Analysis: Flow probe; NADH light guide; Balloon occluder

  19. Multiparameter Analysis: Multi-channel Light Guide; Electrodes (Cae+, Ke+, Nae+; Laser Doppler Tissue Flow; CA flow Probe

  20. Multiparameter Analysis:NADH, Laser Doppler Flow; Ca2e+, K2+

  21. Not the End – Just the Beginning • The story continues through BC’s • Many students • Former colleagues • Friends

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