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Symbols to Know for Crosses. a/a – a is the allele and / represents the two chromatids – there are two alleles for a diploid organism a b/a b – two different genes on the same chromosome a/a ; b/b – two different genes on different chromosomes ; designated separate chromosomes
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Symbols to Know for Crosses • a/a – a is the allele and / represents the two chromatids – there are two alleles for a diploid organism • a b/a b – two different genes on the same chromosome • a/a ; b/b – two different genes on different chromosomes ; designated separate chromosomes • X – symbolizes mating between two different individuals • X - symbolizes a self cross – when the hermaphrodite worms fertilize their own eggs • + is the wild type or non-mutant form of a gene
Hermaphrodite Parent genotype dpy/dpy; +/+ +/+ ; +/0 Male Parent genotype X Creating a heterozygous male Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios Which progeny are you continuing with? Why?
Linkage 1: Cross with Unlinked unc Hermaphrodite Parent genotype +/+ ; unc/unc Male Parent genotype X Female gametes Male gametes F1 Progeny genotype and ratios F1 Progeny phenotypes
Linkage 2: Selfing of hermaphrodite from unlinked cross hermaphrodite self cross X gametes Progeny genotype Progeny phenotypes and ratios
Linkage 1: Crossing with Linked unc Hermaphrodite Parent genotype Male Parent genotype + unc/+ unc X Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios Which progeny are you continuing with? Why?
Linkage 2: Selfing of hermaphrodite from linked cross hermaphrodite self cross X gametes Progeny genotype Progeny phenotypes and ratios