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Chem/Biol 474 W 2008

Chem/Biol 474 W 2008. Instructors: M/W Gerry Prody CB 444 gerry@chem.wwu.edu Paul Craig craigp@cc.wwu.edu T/R Clint Spiegel CB 443 spiegel@wwu.edu Anna Asmudsen. Course Overview. Lab Notebooks. Prelab/Research Project/Paper. Lab Etiquette.

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Chem/Biol 474 W 2008

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  1. Chem/Biol 474 W 2008

  2. Instructors: M/W Gerry Prody CB 444 gerry@chem.wwu.edu Paul Craig craigp@cc.wwu.edu T/R Clint Spiegel CB 443 spiegel@wwu.edu Anna Asmudsen

  3. Course Overview Lab Notebooks Prelab/Research Project/Paper Lab Etiquette Background: Everything in 471 (and 2) is fair game.

  4. BCX-a xylanase from Bacillus cirulans What’s a xylanase? How would you isolate it?

  5. Enzymatic plant cell wall degradation occurs in a wide variety of processes. Firstly, in planta, the enzymatic modification of the cell wall is required for both cell expansion and for developmental events. Secondly, in order to invade a plant host, microorganisms are armed with a deconstructing cocktail of enzymes that depolymerize polysaccharides in the plant cell wall. And thirdly, cell wall degradation is a key process in many industrial applications and in the utilization of plant biomass.

  6. EcoRI Tetracycline Resistance Ampicillin Resistance PstI HindIII + + + + + Origin of Replication A plasmid

  7. Figure 5-43 The pUC18 cloning vector. Page 106

  8. Figure 5-46 Construction of a recombinant DNA molecule. Page 108

  9. Figure 31-1 The induction kinetics of b-galactosidase in E. coli. Page 1217

  10. The lac operon • E-coli uses three enzymes to take up and metabolize lactose. • The genes that code for these three enzymes are clustered on a single operon – the lac Operon. What’s lactose??

  11. Figure 31-2 Genetic map of the E. coli lac operon. Page 1218

  12. Isopropyl thio - -D-galactoside

  13. BCX structure

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