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New Products 소개

New Products 소개. Pel-Freez HLA-B*27 High Resolution Kit (SSP) Pel-Freez KIR Genotyping Kit (SSP) Pel-Freez Cytokine Genotyping Kit (SSP) Dynal SeCore (Sequence based typing). HLA-B*27 High Resolution. HLA-B*27 과 강직성 척추염 1. 강직성 척추염 ; 만성 진행성 다발성 관절염의 일종

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New Products 소개

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  1. New Products 소개 Pel-Freez HLA-B*27 High Resolution Kit (SSP) Pel-Freez KIR Genotyping Kit (SSP) Pel-Freez Cytokine Genotyping Kit (SSP) Dynal SeCore (Sequence based typing)

  2. HLA-B*27 High Resolution • HLA-B*27과 강직성 척추염 • 1. 강직성 척추염 • ; 만성 진행성 다발성 관절염의 일종 • ; 드물게 척추외상, 상부위장관출혈, 대동맥폐쇄부전증, 호흡부전, 아밀로이드성 신증 등의 합병증 발생 위험 • 2. 따라서 조기 발견과 치료가 중요 • 3. HLA-B*2705, 2402, 2704 3개의 아형이 강직성 척추염과 연관되었다고 보고 (Ramos and Lopez. HLA-B27 and spondyloarthritis. Tissue Antigens 2002:60:191~205.) • 4. 보험수가 • - B27 DNA (High Resolution) : 60,760원 • - B27 DNA (Low Resolution) : 28,400원 • - B27 Serology : 45,620원

  3. HLA-B*27 High Resolution • Pel-Freez HLA-B*27 Kit • 1. Kit • 2. 특징 • - Hot Start PCR methods 채용으로 Fidelity 높임 • - Ready to Use Format • - Premixed master mix • - Fast • - Easy Interpretation (UniMatch Plus interpretation software)

  4. KIR Genotyping • NK Cell (Natural Killer Cell) • 체내에서 MHC Class I이 결핍된 암세포 죽이고 전이 억제 • MHC class I에 특이적으로 결합하는 억제 수용체에 의해 NK cell의 능력 부여 • KIR는 이 억제 수용체의 하나

  5. KIR Genotyping • KIR (Killer cell immunoglobulin-like receptor) • 암세포를 죽이고, 전이를 억제하는데 관여 (혈액종양내과) • 최근의 연구에서, - allogenic bone marrow transplantation (BMT) rejection 및 - Graft Versus-host desease (GVHD)와 연관이 있다고 보고

  6. KIR Genotyping Pel-Freez KIR Genotyping 1. Kit 2. 특징 - Hot Start PCR methods 채용으로 Fidelity 높임 - Ready to Use Format - Premixed master mix - Fast - Easy Interpretation (UniMatch Plus interpretation software)

  7. KIR Genotyping Pel-Freez KIR Genotyping 3. KIR Allele Specificity

  8. 3DL3*001/00201/00202/003/004 2DL5A*001, 2DL5B*002-004 3DP1*001/002/00301/00302 2DS4*00101/00102/002 2DS3*00101-00103 2DS4*003 (KIR1D) 2DP1*001/002014 2DL5B*002-004 2DL4*001-007 2DL1*001-005 2DS2*001-005 2DL2*001-004 2DS1*001-004 2DL3*001-006 3DL2*001-012 2DS5*001-003 3DL1*001-009 3DS1*010-014 2DL5A*001 Negative IHW1175 IHW1181 1 2 3 4 5 6 7 8 M 9 10 11 12 13 14 15 16 17 18 19 20 Well # NK Panel Family 1416 Samples Amplified on MJ Tetrad KIR Genotyping

  9. Cytokine Genotyping • 왜 Cytokine Genotyping이 필요한가? • Cytokine Gene polymorphisms - cytokine의 plasma level과 관련 ; kidney, heart 또는 lung allografts에서 생존률과 관련 ; hematopoietic stem cell transplantation에서 GVHD 발생과 관련 • 이식 후 유도 생산 되는 cytokine의 양에 따라 거부반응의 빈도가 좌우됨 • Cytokine의 생성량은 유전형에 따라 다름

  10. Cytokine Genotyping • Which Cytokine? • - IL1 alpha - IL1 beta • - IL1R - IL1RA • - IL2 - IL4 • - IL4R - IL6 • - IL10 - IL12 • - TGF b1 - TNF a • - IFN g

  11. Cytokine Genotyping TGF-beta1 Courtesy J.Mytilineos • 이식 후 TGF-beta1의 생성량은 TGF-beta1의 genotype에 의해 결정 • C (Codon10) G (Codon25)가 보다 높은 TGF-beta1 생성 • TGF-beta1의 생성량이 높을수록 이식 후 예후 불량 • ; TGF-β1 genotyping in kidney transplantation • (Korean J Clin Pathol 2001;21:225-30)

  12. Cytokine Genotyping Pel-Freez Cytokine Genotyping 1. Kit 2. 특징 - Hot Start PCR methods 채용으로 Fidelity 높임 - Ready to Use Format - Premixed master mix - Fast - Easy Interpretation (UniMatch Plus interpretation software)

  13. Cytokine Genotyping Pel-Freez Cytokine Gene Allele Frequencies

  14. Cytokine Genotyping Cytokine Gene Allele Frequencies

  15. SeCore(Sequence Based Genotyping) • 왜 Sequence Based Genogyping이 필요한가? • High Resolution Genotyping 가능 • Ambiguity 해결의 필요성 • 비혈연 골수이식의 증가 • 새로운 대립유전자 발견

  16. SeCore(Sequence Based Genotyping) • SeCore의 장점 • Equipment Compatible - Multi-dye capillary system • Identical Cycling Profiles for all Loci - 1 standard amplification program - 1 sequencing program ; reducing errors and improving efficiency • Amplification Internal Controls - Supply control DNA for trouble shooting

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