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K4.2

IMMUNOGLOBULIN CLASSES. k : 95% in mouse k : 60% in human. K4.2. Different immunoglobulin and their function. Opsonisation agglutination neutralisation antibody-dependent cell-mediated cytotoxicity complement activation mast cell degranulation protection of the neonate.

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K4.2

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  1. IMMUNOGLOBULIN CLASSES k: 95% in mouse k: 60% in human K4.2

  2. Different immunoglobulin and their function Opsonisation agglutination neutralisation antibody-dependent cell-mediated cytotoxicity complement activation mast cell degranulation protection of the neonate

  3. IgG1, IgG2 and IgG4 IgG3 IgG • Structure • Monomer (7S)

  4. IgG • Structure • Properties • Major serum Ig • Major Ig in extravascular spaces • Placental transfer • Fixes complement • Binds to Fc receptors • Phagocytes - opsonization • K cells - ADCC

  5. J Chain CH4 IgM • Structure • Pentamer (19S) • Extra domain (CH4) • J chain

  6. IgM • Structure • Properties • 3rd highest serum Ig • First Ig made by fetus and B cells • Fixes complement

  7. C1r C1r C1s C1s C1q C1q Fixation of C1 by IgG and IgM Abs No activation Activation

  8. Tail Piece IgM • Structure • Properties • 3rd highest serum Ig • First Ig made by fetus and B cells • Fixes complement • Agglutinating Ig • Binds to Fc receptors • B cell surface Ig

  9. Secretory Piece J Chain IgA • Structure • Serum - monomer • Secretions (sIgA) • Dimer (11S) • J chain • Secretory component

  10. IgA • Structure • Properties • 2nd highest serum Ig • Major secretory Ig • Does not fix complement unless aggregated • Binds to Fc receptors on some cells

  11. Tail Piece IgD • Structure • Monomer • Tail piece

  12. IgD • Structure • Properties • 4th highest serum Ig • B cell surface Ig • Does not bind complement

  13. CH4 IgE • Structure • Monomer • Extra domain (CH4)

  14. IgE • Structure • Properties • Least common serum Ig • Allergic reactions • Parasitic infections • Does not fix complement

  15. 14 B. IgG heavy chain variability ~200 variable region segments (V) ~50 diversity regions (D) 4 joining segments (J) 5 constant regions (C)

  16. C. Developmental gene rearrangements 15 DNA of precursor cell (not to scale) V D J C random gene rearrangement B-cell DNA transcription and splicing translation mRNA

  17. A. 19 many antibodies one antibody that recognize that recognizes many epitopes one epitope polyclonal monoclonal making specific specific antibody antibodies requires can be made with purified antigen impure antigen reproducibility difficult various classes one class high reproducibility

  18. 20 B. Monoclonal antibody production B-cells do not survive in culture B-cell fused to myeloma cells produce hybridomas which survive in culture and produce antibody Fusion is inefficient so a selection for hybridomas is used.

  19. immunized mouse 21 fusion mutant myeloma cells antibody producing B-cells cell mixture hybridomas

  20. 22 test supernatant for antibody of interest well containing selective medium dilute retest for antibody of interest

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