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What’s Cooking In Microscopy: A Microscopic Look at the Contents of A Refrigerator

Explore the contents of a refrigerator through the lens of microscopy. This article showcases darkfield and fluorescent microscopy images of shark muscle cells, along with image manipulation using Adobe Photoshop.

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What’s Cooking In Microscopy: A Microscopic Look at the Contents of A Refrigerator

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  1. What’s Cooking In Microscopy:A Microscopic Look at the Contents of A Refrigerator

  2. Darkfield 40x Shark Muscle Cells

  3. DAPI Stained Fluorescent Nuclei Within Shark Muscles

  4. Adobe Photoshop: Adjust Contrast - Brightness

  5. Combined Image Darkfield and Fluorescent Microscopy Done in Layers with Adobe Photoshop

  6. Besides their multinucleated status, shark muscle cells have another aspect, clearly visible the polarized and DIC image below. Both 60x images are of the exact same specimen and clearly show the striations present in shark muscle tissue. Obviously, the two techniques produced dramatically different results. DIC shows slight variations in the muscle surface, while the polarized image shows only the most dramatic aspects of the striations in sharp relief. 60x Polarizer: same image! 60x DIC

  7. Incidentally, the Shark Tissue was triple-stained with fluorescent dyes (10x) Blue Nuclei - DAPI Red Microtubules - Phallicidin Green N-acetyl glucosamine residues in the cells’ surface - WGA

  8. Recrystalized Salt, 40x: Phony Hoffmann Effect – Light Microscope Phase 40 x

  9. Red Leaf Lettuce Focus Through Layers, 100 x Brightfield, oil immersion

  10. Adobe Photoshop Hue / Saturation Adjust

  11. 3 Video Camera Images of Re-crystallized Sugar, 40x Phase Bottom 2 Images: Angled Stage Setting

  12. Phase Image Converted to 8 bit grayscale in image J Look-Up Table Applied To Crystallized Sugar But an Inverted Look-up Table produces dim results! In Photoshop, Contrast and Brightness Adjusted

  13. This Lookup Table Replaces 176 – 255 Red Values with 0. It Creates a Turquoise Effect where Highlights Occurred in the Image for a colored Look at Sugar’s Crystalline Patterns

  14. Onion Cell Nuclei Stained With Iodine - based Fluorescent Stain Random Confocal Slices – Indistinct, Noisy Images… 100 x Magnification

  15. To the left: An Averaged Z-projection in Image J compiling all of the confocal slices. It produced a much cleaner image. And with a little doctoring in Photoshop using the diffuse glow filter and the RGB channel mixer, the nuclei and cell membranes become much more distinct

  16. Brightfield 40 x Image of Same Onion Skin, a little dull …lots of visual goo in the sample. To the right: A simple convolution applied adds a little interest: 0 1 2 -1 1 1 -2 -1 0

  17. But Holy Cow! The convolved onion skin jpeg doctored in Photoshop using Image>Adjust>Curves to give the cell membrane a psychedelic look , crazy man.

  18. Fixing Gretchen… 1. Image J: Image>Adjust>Brightness / Contrast 4. PhotoShop: Filter>Noise>Despeckle 5. PhotoShop: Touch-up with Black and grey “paint” w/paintbrush 2. Image J: Process>Noise>Despeckle 3. PhotoShop: Filter>Smart Blur 6. PhotoShop: Filter>Noise>Add Noise

  19. The End

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